7AWQ

Structure of the thermostabilized EAAT1 cryst-E386Q mutant in complex with L-ASP, sodium ions and the allosteric inhibitor UCPH101

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Excitatory amino acid transporter 1,Neutral amino acid transporter B(0),Excitatory amino acid transporter 1	polymer	522	56511.4	1	UniProt (P43003) UniProt (Q15758) Pfam (PF00375) In PDB	Homo sapiens (Human)	Sodium-dependent glutamate/aspartate transporter 1,GLAST-1,Solute carrier family 1 member 3,ATB(0),Baboon M7 virus receptor,RD114/simian type D retrovirus receptor,Sodium-dependent neutral amino acid transporter type 2,Solute carrier family 1 member 5,Sodium-dependent glutamate/aspartate transporter 1,GLAST-1,Solute carrier family 1 member 3
2	A	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
3	A	ASPARTIC ACID	non-polymer		133.1	1	Chemie (ASP)
4	A	2-Amino-5,6,7,8-tetrahydro-4-(4-methoxyphenyl)-7-(naphthalen-1-yl)-5-oxo-4H-chromene-3-carbonitrile	non-polymer		422.5	1	Chemie (6Z6)
5	A	BARIUM ION	non-polymer		137.3	1	Chemie (BA)

Sequence modifications

A: 1 - 148 (UniProt: P43003)

PDB	External Database	Details
Ser 23	Arg 23	conflict
Phe 44	Tyr 44	conflict
Arg 46	Phe 46	conflict
Leu 50	Phe 50	conflict
Leu 51	Val 51	conflict
Leu 56	Thr 56	conflict
Leu 60	Val 60	conflict
Val 62	Thr 62	conflict
Val 63	Ile 63	conflict
Leu 67	Thr 67	conflict
Pro 72	Arg 72	conflict
Leu 73	Met 73	conflict
Pro 75	Tyr 75	conflict
Ala 82	Ser 82	conflict
Lys 93	Gln 93	conflict
Ile 96	Val 96	conflict
Val 101	Ile 101	conflict
Ile 105	Val 105	conflict
Leu 108	Met 108	conflict
Ser 110	Ala 110	conflict
Ala 113	Ser 113	conflict
Arg 118	Lys 118	conflict
Leu 119	Met 119	conflict
Ser 129	Thr 129	conflict
Leu 137	Ile 137	conflict
Leu 141	Ile 141	conflict
Leu 143	Ile 143	conflict

A: 149 - 223 (UniProt: Q15758)

PDB	External Database	Details
Thr 155	Asn 163	conflict
Cys 175	Ser 183	conflict
Thr 204	Asn 212	conflict

A: 224 - 522 (UniProt: P43003)

PDB	External Database	Details
Val 232	Cys 252	conflict
Ala 236	Val 256	conflict
Leu 237	Ile 257	conflict
Lys 239	Asn 259	conflict
Gly 241	Lys 261	conflict
Leu 246	Ala 266	conflict
Val 248	Arg 268	conflict
Asp 249	Glu 269	conflict
Asn 252	Asp 272	conflict
Thr 258	Ile 278	conflict
Lys 260	Arg 280	conflict
Ile 264	Val 284	conflict
Leu 271	Val 291	conflict
Leu 287	Met 307	conflict
Glu 288	Gly 308	conflict
Leu 290	Ile 310	conflict
Gly 295	Ala 315	conflict
Met 298	Thr 318	conflict
Val 306	Leu 326	conflict
Gly 309	Ala 329	conflict
Leu 310	Val 330	conflict
Ile 316	Leu 336	conflict
Ile 320	Val 340	conflict
Phe 326	Trp 346	conflict
Ala 330	Gly 350	conflict
Ile 332	Leu 352	conflict
Ile 366	Val 386	conflict
Gln 386	Glu 406	conflict
Val 388	Leu 408	conflict
Tyr 399	Phe 419	conflict
Asp 402	Asn 422	conflict
Ala 437	Ser 457	conflict
Leu 454	Phe 474	conflict
Phe 458	Leu 478	conflict
Met 461	Thr 481	conflict
Val 462	Thr 482	conflict
Ala 468	Ser 488	conflict
Lys 480	His 500	conflict
Glu 483	Lys 503	conflict
Lys 484	Asn 504	conflict
Gln 485	Arg 505	conflict
Ala 487	Val 507	conflict
Leu 489	Met 509	conflict

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	56511.4
Non-Polymers*	Number of molecules	5
	Total formula weight	738.9
All*	Total formula weight	57250.3

*Water molecules are not included.