7AUE

Melanocortin receptor 4 (MC4R) Gs protein complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (R)	Melanocortin receptor 4	polymer	350	38972.9	1	UniProt (P32245) Pfam (PF00001)	Homo sapiens (human)	MC4-R
2	B (A)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	373	43194.9	1	UniProt (P63092) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-stimulating G alpha protein
3	C (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	357	39286.9	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
4	D (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
5	E (S)	scFv16	polymer	256	27340.5	1		Mus musculus (Mouse)
6	F (C)	AMINOSERINE	polymer	9	1106.3	1		synthetic construct
7	G (R)	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
8	H (C)	water	water		18.0	1	Chemie (HOH)

Sequence modifications

R: 1 - 332 (UniProt: P32245)

PDB	External Database	Details
Leu 333	-	expression tag
Glu 334	-	expression tag
Val 335	-	expression tag
Leu 336	-	expression tag
Phe 337	-	expression tag
Gln 338	-	expression tag
Gly 339	-	expression tag
Pro 340	-	expression tag
Ser 341	-	expression tag
Gly 342	-	expression tag
Ser 343	-	expression tag
Gly 344	-	expression tag
His 345	-	expression tag
His 346	-	expression tag
His 347	-	expression tag
His 348	-	expression tag
His 349	-	expression tag
His 350	-	expression tag

A: 32 - 373 (UniProt: P63092)

PDB	External Database	Details
Met 1	-	initiating methionine
Gly 2	-	expression tag
Cys 3	-	expression tag
Thr 4	-	expression tag
Leu 5	-	expression tag
Ser 6	-	expression tag
Ala 7	-	expression tag
Glu 8	-	expression tag
Asp 9	-	expression tag
Lys 10	-	expression tag
Ala 11	-	expression tag
Ala 12	-	expression tag
Val 13	-	expression tag
Glu 14	-	expression tag
Arg 15	-	expression tag
Ser 16	-	expression tag
Lys 17	-	expression tag
Met 18	-	expression tag
Ile 19	-	expression tag
Asp 20	-	expression tag
Arg 21	-	expression tag
Asn 22	-	expression tag
Leu 23	-	expression tag
Arg 24	-	expression tag
Glu 25	-	expression tag
Asp 26	-	expression tag
Gly 27	-	expression tag
Glu 28	-	expression tag
Lys 29	-	expression tag
Ala 30	-	expression tag
Ala 31	-	expression tag
-	Glu 71	deletion
-	Gly 72	deletion
-	Gly 73	deletion
-	Glu 74	deletion
-	Glu 75	deletion
-	Asp 76	deletion
-	Pro 77	deletion
-	Gln 78	deletion
-	Ala 79	deletion
-	Ala 80	deletion
-	Arg 81	deletion
-	Ser 82	deletion
-	Asn 83	deletion
-	Ser 84	deletion
Ser 65	Gly 86	conflict

B: 19 - 357 (UniProt: P62873)

PDB	External Database	Details
His 1	-	expression tag
His 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
Leu 7	-	expression tag
Glu 8	-	expression tag
Val 9	-	expression tag
Leu 10	-	expression tag
Phe 11	-	expression tag
Gln 12	-	expression tag
Gly 13	-	expression tag
Pro 14	-	expression tag
Gly 15	-	expression tag
Ser 16	-	expression tag
Ser 17	-	expression tag
Gly 18	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	157762.6
Non-Polymers*	Number of molecules	1
	Total formula weight	40.1
All*	Total formula weight	157802.7

*Water molecules are not included.