7ASZ

L-2-haloacid dehalogenase H190A mutant from Zobellia galactanivorans

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	(S)-2-haloacid dehalogenase	polymer	238	26309.3	2	UniProt (G0L7V6) Pfam (PF00702) In PDB	Zobellia galactanivorans (strain DSM 12802 / CCUG 47099 / CIP 106680 / NCIMB 13871 / Dsij)	L-2-haloacid dehalogenase
2	A, B	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
3	A, B	THIOCYANATE ION	non-polymer		58.1	2	Chemie (SCN)
4		water	water		18.0	407	Chemie (HOH)

Sequence modifications

A, B: 2 - 227 (UniProt: G0L7V6)

PDB	External Database	Details
Met -10	-	initiating methionine
Gly -9	-	expression tag
Ser -8	-	expression tag
Ser -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag
Ala 179	His 179	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	52618.6
Non-Polymers*	Number of molecules	4
	Total formula weight	306.1
All*	Total formula weight	52924.8

*Water molecules are not included.