7ARD

Cryo-EM structure of Polytomella Complex-I (complete composition)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	ND3	polymer	154	17828.6	1		Polytomella sp. Pringsheim 198.80
2	B (B)	PSST	polymer	164	18158.2	1		Polytomella sp. Pringsheim 198.80
3	C (C)	ND9	polymer	217	25689.1	1		Polytomella sp. Pringsheim 198.80
4	D (D)	ND7	polymer	395	45263.0	1		Polytomella sp. Pringsheim 198.80
5	E (E)	24 kDa	polymer	276	30186.5	1		Polytomella sp. Pringsheim 198.80
6	F (F)	51 kDa	polymer	469	51593.9	1		Polytomella sp. Pringsheim 198.80
7	G (G)	75 kDa	polymer	720	77364.3	1		Polytomella sp. Pringsheim 198.80
8	H (H)	ND1	polymer	293	31829.8	1		Polytomella sp. Pringsheim 198.80
9	I (I)	TYKY	polymer	229	26140.5	1		Polytomella sp. Pringsheim 198.80
10	J (J)	ND6	polymer	145	15938.1	1		Polytomella sp. Pringsheim 198.80
11	K (K)	ND4L	polymer	127	13836.1	1		Polytomella sp. Pringsheim 198.80
12	L (L)	ND5	polymer	536	58317.4	1		Polytomella sp. Pringsheim 198.80
13	M (M)	ND4	polymer	438	48407.1	1		Polytomella sp. Pringsheim 198.80
14	N (N)	ND2	polymer	375	41939.4	1		Polytomella sp. Pringsheim 198.80
15	O (O)	C1-FDX	polymer	200	23115.1	1		Polytomella sp. Pringsheim 198.80
16	P (P)	39 kDa	polymer	370	41233.0	1		Polytomella sp. Pringsheim 198.80
17	Q (Q)	18 kDa	polymer	185	20800.8	1		Polytomella sp. Pringsheim 198.80
18	R (R)	13 kDa	polymer	132	14346.2	1		Polytomella sp. Pringsheim 198.80
19	S (S)	B8	polymer	98	10509.9	1		Polytomella sp. Pringsheim 198.80
20	T (T)	SDAP1	polymer	123	13409.2	1		Polytomella sp. Pringsheim 198.80
21	U (U)	SDAP2	polymer	122	13393.1	1		Polytomella sp. Pringsheim 198.80
22	V (V)	B13	polymer	159	17745.2	1		Polytomella sp. Pringsheim 198.80
23	W (W)	B14	polymer	137	16134.9	1		Polytomella sp. Pringsheim 198.80
24	X (X)	PGIV	polymer	100	11758.5	1		Polytomella sp. Pringsheim 198.80
25	Y (Y)	B14.7	polymer	206	22521.6	1		Polytomella sp. Pringsheim 198.80
26	Z (Z)	B16.6	polymer	142	16261.7	1		Polytomella sp. Pringsheim 198.80
27	AA (a)	MWFE	polymer	71	8504.5	1		Polytomella sp. Pringsheim 198.80
28	BA (b)	B9	polymer	54	4613.7	1		Polytomella sp. Pringsheim 198.80
29	CA (c)	KFYI	polymer	110	12554.1	1		Polytomella sp. Pringsheim 198.80
30	DA (d)	B14.5b	polymer	83	9575.0	1		Polytomella sp. Pringsheim 198.80
31	EA (e)	15 kDa	polymer	75	8915.2	1		Polytomella sp. Pringsheim 198.80
32	FA (f)	MNLL	polymer	121	13507.2	1		Polytomella sp. Pringsheim 198.80
33	GA (g)	ESSS	polymer	172	19333.2	1		Polytomella sp. Pringsheim 198.80
34	HA (h)	NUOP4	polymer	81	9145.3	1		Polytomella sp. Pringsheim 198.80
35	IA (i)	NUOP5	polymer	128	15118.8	1		Polytomella sp. Pringsheim 198.80
36	JA (j)	AGGG	polymer	87	7422.1	1		Polytomella sp. Pringsheim 198.80
37	KA (k)	B12	polymer	55	6332.2	1		Polytomella sp. Pringsheim 198.80
38	LA (l)	ASHI	polymer	151	16986.6	1		Polytomella sp. Pringsheim 198.80
39	MA (m)	B15	polymer	138	16276.2	1		Polytomella sp. Pringsheim 198.80
40	NA (n)	Complex I-B22	polymer	121	14418.6	1	UniProt (A0A024FSS5) Pfam (PF05347)	Polytomella sp. Pringsheim 198.80	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9,NADH-ubiquinone oxidoreductase B22 subunit
41	OA (o)	B18	polymer	85	10293.0	1		Polytomella sp. Pringsheim 198.80
42	PA (p)	PDSW	polymer	156	18434.9	1		Polytomella sp. Pringsheim 198.80
43	QA (q)	B17.2	polymer	155	18178.4	1		Polytomella sp. Pringsheim 198.80
44	RA (r)	B14.5a	polymer	121	13827.0	1		Polytomella sp. Pringsheim 198.80
45	SA (s)	NUOP7	polymer	118	13476.5	1		Polytomella sp. Pringsheim 198.80
46	TA (t)	NUOP8	polymer	134	15743.9	1		Polytomella sp. Pringsheim 198.80
47	UA (u)	unknown	polymer	50	4273.3	1		Polytomella sp. Pringsheim 198.80
48	VA (w)	unknown	polymer	41	3507.3	1		Polytomella sp. Pringsheim 198.80
49	WA (x)	CAL	polymer	280	31348.4	1		Polytomella sp. Pringsheim 198.80
50	XA (y)	CA2	polymer	310	33201.9	1		Polytomella sp. Pringsheim 198.80
51	YA (z)	CA3	polymer	227	24150.5	1		Polytomella sp. Pringsheim 198.80
52	EC, LB, PB, QB, SB... (z, L, M, N, A)	(7S)-4-HYDROXY-N,N,N-TRIMETHYL-9-OXO-7-[(PALMITOYLOXY)METHYL]-3,5,8-TRIOXA-4-PHOSPHAHEXACOSAN-1-AMINIUM 4-OXIDE	non-polymer		763.1	7	Chemie (PC7)
53	AB, DB, FB, GB, IB... (B, F, G, I)	IRON/SULFUR CLUSTER	non-polymer		351.6	6	Chemie (SF4)
54	BB, EB (E, G)	FE2/S2 (INORGANIC) CLUSTER	non-polymer		175.8	2	Chemie (FES)
55	CB (F)	FLAVIN MONONUCLEOTIDE	non-polymer		456.3	1	Chemie (FMN)
56	AC, BC, HB, MB, NB... (m, H, L, M, N...)	PHOSPHATIDYLETHANOLAMINE	non-polymer		734.0	7	Chemie (PTY)
57	DC, KB, OB, RB, ZB (t, L, M, N, d)	CARDIOLIPIN	non-polymer		1464.0	5	Chemie (CDL)
58	VB (P)	NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	non-polymer		745.4	1	Chemie (NDP)
59	WB (R)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
60	CC, XB (n, W)	S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate	non-polymer		540.7	2	Chemie (8Q1)
61	AD, BD, CD, DD, ED... (W, X, Y, Z, a...)	water	water		18.0	782	Chemie (HOH)

Contents of the asymmetric unit

Polymers	Number of chains	51
	Total formula weight	1072858.9
Non-Polymers*	Number of molecules	32
	Total formula weight	22610.1
All*	Total formula weight	1095469.1

*Water molecules are not included.