7ARB
Cryo-EM structure of Arabidopsis thaliana Complex-I (complete composition)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | NADH-ubiquinone oxidoreductase chain 3 | polymer | 119 | 13941.4 | 1 | UniProt (P92533) Pfam (PF00507) | Arabidopsis thaliana (Mouse-ear cress) | NADH dehydrogenase subunit 3 |
| 2 | B (B) | NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial | polymer | 218 | 24071.9 | 1 | UniProt (Q42577) Pfam (PF01058) | Arabidopsis thaliana (Mouse-ear cress) | |
| 3 | C (C) | NADH dehydrogenase [ubiquinone] iron-sulfur protein 3 | polymer | 190 | 22910.9 | 1 | UniProt (Q95748) Pfam (PF00329) | Arabidopsis thaliana (Mouse-ear cress) | NADH dehydrogenase subunit 9 |
| 4 | D (D) | NADH dehydrogenase subunit 7 | polymer | 394 | 45036.8 | 1 | UniProt (A0A2P2CLH2) Pfam (PF00346) UniProt (by SIFTS) (P93306) | Arabidopsis thaliana (Mouse-ear cress) | |
| 5 | E (E) | NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial | polymer | 255 | 28423.6 | 1 | UniProt (O22769) Pfam (PF01257) | Arabidopsis thaliana (Mouse-ear cress) | |
| 6 | F (F) | NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial | polymer | 486 | 53522.4 | 1 | UniProt (Q9FNN5) Pfam (PF01512) Pfam (PF22461) Pfam (PF10589) | Arabidopsis thaliana (Mouse-ear cress) | |
| 7 | G (G) | NADH dehydrogenase [ubiquinone] iron-sulfur protein 1, mitochondrial | polymer | 748 | 81619.4 | 1 | UniProt (Q9FGI6) Pfam (PF10588) Pfam (PF22117) Pfam (PF22151) Pfam (PF00384) Pfam (PF09326) Pfam (PF13510) | Arabidopsis thaliana (Mouse-ear cress) | Protein EMBRYO DEFECTIVE 1467 |
| 8 | H (H) | NADH-ubiquinone oxidoreductase chain 1 | polymer | 325 | 36020.1 | 1 | UniProt (B5TM92) Pfam (PF00146) UniProt (by SIFTS) (P92558) | Arabidopsis thaliana (Mouse-ear cress) | |
| 9 | I (I) | NADH dehydrogenase [ubiquinone] iron-sulfur protein 8-A, mitochondrial | polymer | 222 | 25536.8 | 1 | UniProt (Q42599) Pfam (PF12838) | Arabidopsis thaliana (Mouse-ear cress) | |
| 10 | J (J) | NADH-ubiquinone oxidoreductase chain 6 | polymer | 205 | 23690.4 | 1 | UniProt (A0A2P2CLG1) Pfam (PF00499) UniProt (by SIFTS) (P60497) | Arabidopsis thaliana (Mouse-ear cress) | |
| 11 | K (K) | NADH dehydrogenase subunit 4L | polymer | 100 | 11165.5 | 1 | UniProt (A0A2P2CLH7) Pfam (PF00420) UniProt (by SIFTS) (Q04614) | Arabidopsis thaliana (Mouse-ear cress) | |
| 12 | L (L) | NADH-ubiquinone oxidoreductase chain 5 | polymer | 669 | 74498.0 | 1 | UniProt (B5TM94) Pfam (PF00662) Pfam (PF00361) Pfam (PF06455) UniProt (by SIFTS) (P29388) | Arabidopsis thaliana (Mouse-ear cress) | |
| 13 | M (M) | NADH-ubiquinone oxidoreductase chain 4 | polymer | 495 | 55995.7 | 1 | UniProt (B5TM93) Pfam (PF00361) UniProt (by SIFTS) (P93313) | Arabidopsis thaliana (Mouse-ear cress) | |
| 14 | N (N) | NADH-ubiquinone oxidoreductase chain 2 | polymer | 499 | 55486.8 | 1 | UniProt (O05000) Pfam (PF00361) | Arabidopsis thaliana (Mouse-ear cress) | NADH dehydrogenase subunit 2 |
| 15 | O (O) | AT3G07480.1 | polymer | 159 | 17626.2 | 1 | UniProt (A0A384LA38) UniProt (by SIFTS) (Q9SRR8) | Arabidopsis thaliana (Mouse-ear cress) | |
| 16 | P (P) | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial | polymer | 402 | 43988.7 | 1 | UniProt (Q9SK66) Pfam (PF01370) | Arabidopsis thaliana (Mouse-ear cress) | |
| 17 | Q (Q) | NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial | polymer | 154 | 17160.4 | 1 | UniProt (Q9FJW4) Pfam (PF04800) | Arabidopsis thaliana (Mouse-ear cress) | Protein FROSTBITE1 |
| 18 | R (R) | NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial | polymer | 110 | 12251.1 | 1 | UniProt (Q9M9M6) Pfam (PF10276) | Arabidopsis thaliana (Mouse-ear cress) | |
| 19 | S (S) | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 | polymer | 97 | 10865.8 | 1 | UniProt (Q9FIJ2) Pfam (PF05047) | Arabidopsis thaliana (Mouse-ear cress) | |
| 20 | T (T) | Acyl carrier protein 1, mitochondrial | polymer | 122 | 13735.6 | 1 | UniProt (P53665) Pfam (PF00550) | Arabidopsis thaliana (Mouse-ear cress) | MtACP-1,ACP,NADH-ubiquinone oxidoreductase 9.6 kDa subunit |
| 21 | U (U) | Acyl carrier protein 2, mitochondrial | polymer | 126 | 14183.1 | 1 | UniProt (O80800) Pfam (PF00550) | Arabidopsis thaliana (Mouse-ear cress) | MtACP-2,ACP,NADH-ubiquinone oxidoreductase 9.6 kDa subunit |
| 22 | V (V) | Probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochondrial | polymer | 169 | 19201.9 | 1 | UniProt (Q9FLX7) Pfam (PF04716) | Arabidopsis thaliana (Mouse-ear cress) | |
| 23 | W (W) | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 | polymer | 133 | 15102.3 | 1 | UniProt (Q9LHI0) | Arabidopsis thaliana (Mouse-ear cress) | |
| 24 | X (X) | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8-B | polymer | 106 | 11986.0 | 1 | UniProt (Q8LGE7) Pfam (PF06747) | Arabidopsis thaliana (Mouse-ear cress) | |
| 25 | Y (Z) | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13-A | polymer | 143 | 16145.6 | 1 | UniProt (Q8RWA7) Pfam (PF06212) | Arabidopsis thaliana (Mouse-ear cress) | Protein MATERNAL EFFECT EMBRYO ARREST 4 |
| 26 | Z (a) | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 | polymer | 65 | 7349.6 | 1 | UniProt (Q9C9Z5) Pfam (PF15879) | Arabidopsis thaliana (Mouse-ear cress) | |
| 27 | AA (b) | At2g46540/F11C10.23 | polymer | 65 | 6810.2 | 1 | UniProt (Q9ZPY5) | Arabidopsis thaliana (Mouse-ear cress) | Expressed protein,F11C10.23/F11C10.23,Fiber |
| 28 | BA (c) | Transmembrane protein | polymer | 88 | 9876.4 | 1 | UniProt (Q8VZT9) | Arabidopsis thaliana (Mouse-ear cress) | KFYI -- 1 subunit C1 |
| 29 | CA (d) | Excitatory amino acid transporter | polymer | 81 | 9220.7 | 1 | UniProt (Q94AL6) | Arabidopsis thaliana (Mouse-ear cress) | |
| 30 | DA (e) | NADH dehydrogenase [ubiquinone] iron-sulfur protein 5-B | polymer | 83 | 9914.1 | 1 | UniProt (Q9LZI6) Pfam (PF10200) | Arabidopsis thaliana (Mouse-ear cress) | |
| 31 | EA (f) | At4g16450 | polymer | 106 | 11355.0 | 1 | UniProt (Q84W12) Pfam (PF10785) | Arabidopsis thaliana (Mouse-ear cress) | NADH-ubiquinone oxidoreductase |
| 32 | FA (g) | ESSS subunit of NADH:ubiquinone oxidoreductase (Complex I) protein | polymer | 114 | 12648.3 | 1 | UniProt (Q9SLC8) | Arabidopsis thaliana (Mouse-ear cress) | |
| 33 | GA (i) | P1 | polymer | 98 | 11808.2 | 1 | UniProt (A0A178W1I8) Pfam (PF17250) UniProt (by SIFTS) (Q9FYF8) | Arabidopsis thaliana (Mouse-ear cress) | |
| 34 | HA (j) | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2 | polymer | 69 | 7582.6 | 1 | UniProt (Q8LDK3) | Arabidopsis thaliana (Mouse-ear cress) | |
| 35 | IA (k) | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3-A | polymer | 72 | 8064.3 | 1 | UniProt (O64725) Pfam (PF08122) | Arabidopsis thaliana (Mouse-ear cress) | |
| 36 | JA (l) | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial | polymer | 125 | 13225.2 | 1 | UniProt (Q9FGK0) | Arabidopsis thaliana (Mouse-ear cress) | |
| 37 | KA (m) | B15 -- 1 beta subcomplex subunit 4 | polymer | 71 | 8305.5 | 1 | UniProt (A0A178VZI4) UniProt (by SIFTS) (Q9SIQ8) | Arabidopsis thaliana (Mouse-ear cress) | |
| 38 | LA (n) | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 | polymer | 117 | 13638.3 | 1 | UniProt (Q945M1) | Arabidopsis thaliana (Mouse-ear cress) | B22 subunit of eukaryotic mitochondrial complex I,Complex I-B22,CI-B22,NADH-ubiquinone oxidoreductase B22 subunit |
| 39 | MA (o) | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 | polymer | 103 | 11757.8 | 1 | UniProt (Q9SKC9) Pfam (PF05676) | Arabidopsis thaliana (Mouse-ear cress) | |
| 40 | NA (p) | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10-B | polymer | 106 | 12462.3 | 1 | UniProt (Q94C12) Pfam (PF10249) | Arabidopsis thaliana (Mouse-ear cress) | |
| 41 | OA (q) | Probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 | polymer | 159 | 18346.7 | 1 | UniProt (Q9M9M9) Pfam (PF05071) | Arabidopsis thaliana (Mouse-ear cress) | |
| 42 | PA (r) | Furry | polymer | 131 | 15060.1 | 1 | UniProt (Q9SD78) | Arabidopsis thaliana (Mouse-ear cress) | |
| 43 | QA (u) | unknown | polymer | 30 | 2571.2 | 1 | Arabidopsis thaliana | ||
| 44 | RA (v) | Uncharacterized protein At2g27730, mitochondrial | polymer | 113 | 11965.5 | 1 | UniProt (Q9ZUX4) | Arabidopsis thaliana (Mouse-ear cress) | |
| 45 | SA (x) | Gamma carbonic anhydrase-like 2, mitochondrial | polymer | 256 | 27985.1 | 1 | UniProt (Q9SMN1) | Arabidopsis thaliana (Mouse-ear cress) | GAMMA CAL2 |
| 46 | TA (y) | Gamma carbonic anhydrase 2, mitochondrial | polymer | 278 | 30102.2 | 1 | UniProt (Q9C6B3) Pfam (PF00132) | Arabidopsis thaliana (Mouse-ear cress) | GAMMA CA2,Transcription factor APFI |
| 47 | UA (z) | Gamma carbonic anhydrase 1, mitochondrial | polymer | 275 | 30010.0 | 1 | UniProt (Q9FWR5) | Arabidopsis thaliana (Mouse-ear cress) | GAMMA CA1 |
| 48 | BB, CB, EB, FB, VA... (G, I, B, F) | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 6 | Chemie (SF4) | |||
| 49 | GB, IB, WA (M, N, D) | PHOSPHATIDYLETHANOLAMINE | non-polymer | 734.0 | 3 | Chemie (PTY) PubChem (446872) PubChem (3330137) | |||
| 50 | AB, XA (G, E) | FE2/S2 (INORGANIC) CLUSTER | non-polymer | 175.8 | 2 | Chemie (FES) | |||
| 51 | YA (F) | FLAVIN MONONUCLEOTIDE | non-polymer | 456.3 | 1 | Chemie (FMN) PubChem (710) PubChem (23585816) PubChem (8965) PubChem (51435500) PubChem (51435503) PubChem (643976) PubChem (5702760) PubChem (7048779) PubChem (7048781) PubChem (15559251) | |||
| 52 | DB (H) | Ubiquinone-9 | non-polymer | 795.2 | 1 | Chemie (UQ9) PubChem (99490) PubChem (5280473) PubChem (97038296) PubChem (97038294) PubChem (97038295) PubChem (125181414) PubChem (137350099) | |||
| 53 | HB (M) | Lauryl Maltose Neopentyl Glycol | non-polymer | 1005.2 | 1 | Chemie (LMN) PubChem (49839603) PubChem (70678409) PubChem (75300949) PubChem (132285124) PubChem (153502978) PubChem (126731017) PubChem (172914192) | |||
| 54 | JB (O) | FE (III) ION | non-polymer | 55.8 | 1 | Chemie (FE) PubChem (29936) | |||
| 55 | KB (P) | NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE | non-polymer | 745.4 | 1 | Chemie (NDP) | |||
| 56 | LB, SB (R, y) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 57 | MB, QB (W, n) | S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate | non-polymer | 540.7 | 2 | Chemie (8Q1) PubChem (119058169) PubChem (122362359) PubChem (162826280) | |||
| 58 | NB, RB (c, v) | (7S)-4-HYDROXY-N,N,N-TRIMETHYL-9-OXO-7-[(PALMITOYLOXY)METHYL]-3,5,8-TRIOXA-4-PHOSPHAHEXACOSAN-1-AMINIUM 4-OXIDE | non-polymer | 763.1 | 2 | Chemie (PC7) PubChem (14389436) PubChem (449006) PubChem (5313171) | |||
| 59 | OB (f) | (1S)-2-{[{[(2R)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL STEARATE | non-polymer | 751.0 | 1 | Chemie (PGT) PubChem (25267633) PubChem (449008) PubChem (102447212) PubChem (16040248) PubChem (172432040) | |||
| 60 | PB (f) | Phosphatidylinositol | non-polymer | 887.1 | 1 | Chemie (T7X) PubChem (42607491) PubChem (21226108) PubChem (59423079) PubChem (71581204) PubChem (174696) PubChem (72280035) PubChem (101025742) PubChem (101668931) PubChem (102207642) PubChem (6441989) PubChem (10260108) PubChem (134744895) PubChem (123819699) PubChem (154815683) PubChem (162368813) PubChem (137350037) PubChem (162371041) PubChem (172878298) | |||
| 61 | TB (z) | 1,2-DICAPROYL-SN-PHOSPHATIDYL-L-SERINE | non-polymer | 455.4 | 1 | Chemie (PSF) PubChem (17754227) PubChem (44354119) PubChem (101771308) PubChem (9547083) PubChem (9547088) PubChem (15409343) |
Sequence modifications
D: 1 - 394 (UniProt: A0A2P2CLH2)
L: 1 - 669 (UniProt: B5TM94)
M: 1 - 495 (UniProt: B5TM93)
| PDB | External Database | Details |
|---|---|---|
| Ser 363 | Leu 363 | variant |
| PDB | External Database | Details |
|---|---|---|
| Phe 91 | Ser 91 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Leu 326 | Pro 326 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 47 |
| Total formula weight | 1034226.0 | |
| Non-Polymers* | Number of molecules | 25 |
| Total formula weight | 12553.5 | |
| All* | Total formula weight | 1046779.5 |
