7AQU

Flavin-dependent tryptophan halogenase Thal: N-terminally His-tagged form of quintuple mutant (NHis-Thal-RebH5)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Tryptophan 6-halogenase	polymer	551	62362.3	2	UniProt (A1E280) Pfam (PF04820) In PDB	Streptomyces albogriseolus
2	A	BICINE	non-polymer		163.2	1	Chemie (BCN)
3	A, B	GLYCEROL	non-polymer		92.1	5	Chemie (GOL)
4	A, B	GLYCINE	non-polymer		75.1	7	Chemie (GLY)
5	A	ALANINE	non-polymer		89.1	1	Chemie (ALA)
6	A	SERINE	non-polymer		105.1	1	Chemie (SER)
7		water	water		18.0	975	Chemie (HOH)

Sequence modifications

A, B: 1 - 531 (UniProt: A1E280)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ile 52	Val 52	engineered mutation
Ile 82	Val 82	engineered mutation
Thr 360	Ser 360	engineered mutation
Ser 469	Gly 469	engineered mutation
Asn 470	Ser 470	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	124724.6
Non-Polymers*	Number of molecules	15
	Total formula weight	1343.3
All*	Total formula weight	126067.9

*Water molecules are not included.