7AQA

Pseudomonas stutzeri nitrous oxide reductase mutant, H382A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Nitrous-oxide reductase	polymer	646	71891.5	2	UniProt (P19573) Pfam (PF18793) Pfam (PF18764) Pfam (PF00116)	Pseudomonas stutzeri	N(2)OR,N2O reductase
2	C (A)	sulfanyl-(tricuprio-$l^{4}-sulfanyl)copper	non-polymer		319.3	1	Chemie (K7E)
3	D, M (A, B)	2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		282.3	2	Chemie (B3P)
4	E (A)	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
5	F, N (A, B)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
6	G, O (A, B)	POTASSIUM ION	non-polymer		39.1	2	Chemie (K)
7	H, I, Q (A, B)	CHLORIDE ION	non-polymer		35.5	3	Chemie (CL)
8	J, R (A, B)	DINUCLEAR COPPER ION	non-polymer		127.1	2	Chemie (CUA)
9	K (B)	PHENYLALANINE	non-polymer		165.2	1	Chemie (PHE)
10	L (B)	(dicuprio-$l^{3}-sulfanyl)-sulfanyl-copper	non-polymer		255.8	1	Chemie (RUQ)
11	P (B)	FORMIC ACID	non-polymer		46.0	1	Chemie (FMT)
12	S, T (A, B)	water	water		18.0	1088	Chemie (HOH)

Sequence modifications

A, B: 1 - 638 (UniProt: P19573)

PDB	External Database	Details
Ala 382	His 382	engineered mutation
Trp 639	-	expression tag
Ser 640	-	expression tag
His 641	-	expression tag
Pro 642	-	expression tag
Gln 643	-	expression tag
Phe 644	-	expression tag
Glu 645	-	expression tag
Lys 646	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	143783.0
Non-Polymers*	Number of molecules	16
	Total formula weight	1892.9
All*	Total formula weight	145675.8

*Water molecules are not included.