7AQ8

Pseudomonas stutzeri nitrous oxide reductase mutant, H583Y/D576A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Nitrous-oxide reductase	polymer	646	71939.6	2	UniProt (P19573) Pfam (PF18793) Pfam (PF18764) Pfam (PF00116)	Pseudomonas stutzeri	N(2)OR,N2O reductase
2	C, R (A, B)	2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		282.3	2	Chemie (B3P)
3	D, S (A, B)	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
4	E, T (A, B)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
5	F, U (A, B)	POTASSIUM ION	non-polymer		39.1	2	Chemie (K)
6	AA, G, H, I, J... (B, A)	FORMIC ACID	non-polymer		46.0	9	Chemie (FMT)
7	K, X (A, B)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
8	L, Z (A, B)	DINUCLEAR COPPER ION	non-polymer		127.1	2	Chemie (CUA)
9	M, N (A)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
10	P (A)	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	1	Chemie (TRS)
11	Y (B)	(MU-4-SULFIDO)-TETRA-NUCLEAR COPPER ION	non-polymer		286.2	1	Chemie (CUZ)
12	BA, CA (A, B)	water	water		18.0	756	Chemie (HOH)

Sequence modifications

A, B: 1 - 638 (UniProt: P19573)

PDB	External Database	Details
Ala 576	Asp 576	conflict
Tyr 583	His 583	conflict
Trp 639	-	expression tag
Ser 640	-	expression tag
His 641	-	expression tag
Pro 642	-	expression tag
Gln 643	-	expression tag
Phe 644	-	expression tag
Glu 645	-	expression tag
Lys 646	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	143879.1
Non-Polymers*	Number of molecules	25
	Total formula weight	2047.5
All*	Total formula weight	145926.7

*Water molecules are not included.