7AQ7
Pseudomonas stutzeri nitrous oxide reductase mutant, H583Y
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B | Nitrous-oxide reductase | polymer | 646 | 71983.6 | 2 | UniProt (P19573) Pfam (PF18793) Pfam (PF18764) Pfam (PF00116) In PDB | Pseudomonas stutzeri | N(2)OR,N2O reductase |
| 2 | A, B | 2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 282.3 | 2 | Chemie (B3P) | |||
| 3 | A, B | SODIUM ION | non-polymer | 23.0 | 2 | Chemie (NA) | |||
| 4 | A, B | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
| 5 | A, B | POTASSIUM ION | non-polymer | 39.1 | 2 | Chemie (K) | |||
| 6 | B, A | FORMIC ACID | non-polymer | 46.0 | 11 | Chemie (FMT) | |||
| 7 | A, B | CHLORIDE ION | non-polymer | 35.5 | 3 | Chemie (CL) | |||
| 8 | A, B | (MU-4-SULFIDO)-TETRA-NUCLEAR COPPER ION | non-polymer | 286.2 | 2 | Chemie (CUZ) | |||
| 9 | A, B | DINUCLEAR COPPER ION | non-polymer | 127.1 | 2 | Chemie (CUA) | |||
| 10 | A | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 122.1 | 1 | Chemie (TRS) | |||
| 11 | water | water | 18.0 | 940 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 638 (UniProt: P19573)
| PDB | External Database | Details |
|---|---|---|
| Tyr 583 | His 583 | engineered mutation |
| Trp 639 | - | expression tag |
| Ser 640 | - | expression tag |
| His 641 | - | expression tag |
| Pro 642 | - | expression tag |
| Gln 643 | - | expression tag |
| Phe 644 | - | expression tag |
| Glu 645 | - | expression tag |
| Lys 646 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 143967.2 | |
| Non-Polymers* | Number of molecules | 27 |
| Total formula weight | 2330.5 | |
| All* | Total formula weight | 146297.6 |
