7AQ4

Pseudomonas stutzeri nitrous oxide reductase mutant, H583E

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Nitrous-oxide reductase	polymer	646	71949.5	2	UniProt (P19573) Pfam (PF18793) Pfam (PF18764) Pfam (PF00116)	Pseudomonas stutzeri	N(2)OR,N2O reductase
2	C, Q (A, B)	2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		282.3	2	Chemie (B3P)
3	D, R (A, B)	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
4	E, S (A, B)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
5	AA, F, G, H, I... (B, A)	FORMIC ACID	non-polymer		46.0	10	Chemie (FMT)
6	J, X (A, B)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
7	K, Y (A, B)	DINUCLEAR COPPER ION	non-polymer		127.1	2	Chemie (CUA)
8	M, N (A)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
9	O (A)	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	1	Chemie (TRS)
10	T (B)	POTASSIUM ION	non-polymer		39.1	1	Chemie (K)
11	Z (B)	(MU-4-SULFIDO)-TETRA-NUCLEAR COPPER ION	non-polymer		286.2	1	Chemie (CUZ)
12	BA, CA (A, B)	water	water		18.0	1069	Chemie (HOH)

Sequence modifications

A, B: 1 - 638 (UniProt: P19573)

PDB	External Database	Details
Glu 583	His 583	engineered mutation
Trp 639	-	expression tag
Ser 640	-	expression tag
His 641	-	expression tag
Pro 642	-	expression tag
Gln 643	-	expression tag
Phe 644	-	expression tag
Glu 645	-	expression tag
Lys 646	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	143899.0
Non-Polymers*	Number of molecules	25
	Total formula weight	2054.5
All*	Total formula weight	145953.5

*Water molecules are not included.