7AQ0

Pseudomonas stutzeri nitrous oxide reductase mutant, D576A/S550A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Nitrous-oxide reductase	polymer	645	71769.4	2	UniProt (P19573) Pfam (PF18793) Pfam (PF18764) Pfam (PF00116)	Pseudomonas stutzeri	N(2)OR,N2O reductase
2	C, P (A, B)	2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		282.3	2	Chemie (B3P)
3	D, Q (A, B)	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
4	E, R (A, B)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
5	F, G, H, L, T... (A, B)	FORMIC ACID	non-polymer		46.0	8	Chemie (FMT)
6	I, V (A, B)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
7	J, W (A, B)	(MU-4-SULFIDO)-TETRA-NUCLEAR COPPER ION	non-polymer		286.2	2	Chemie (CUZ)
8	K, X (A, B)	DINUCLEAR COPPER ION	non-polymer		127.1	2	Chemie (CUA)
9	M (A)	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	1	Chemie (TRS)
10	N, S (A, B)	POTASSIUM ION	non-polymer		39.1	2	Chemie (K)
11	O (B)	PHENYLALANINE	non-polymer		165.2	1	Chemie (PHE)
12	AA, BA (A, B)	water	water		18.0	918	Chemie (HOH)

Sequence modifications

A, B: 1 - 638 (UniProt: P19573)

PDB	External Database	Details
Ala 550	Ser 550	engineered mutation
Ala 576	Asp 576	engineered mutation
Trp 639	-	expression tag
Ser 640	-	expression tag
His 641	-	expression tag
Pro 642	-	expression tag
Gln 643	-	expression tag
Phe 644	-	expression tag
Glu 645	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	143538.7
Non-Polymers*	Number of molecules	24
	Total formula weight	2322.1
All*	Total formula weight	145860.9

*Water molecules are not included.