7AMU

Crystal structure of rsEGFP2 T204A in its fluorescent on-state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Green fluorescent protein	polymer	252	28502.2	1	UniProt (P42212) Pfam (PF01353) In PDB	Aequorea victoria
2	A	GLYCEROL	non-polymer		92.1	6	Chemie (GOL)
3	A	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
4		water	water		18.0	174	Chemie (HOH)

Sequence modifications

A: 3 - 239 (UniProt: P42212)

PDB	External Database	Details
Met -12	-	initiating methionine
Arg -11	-	expression tag
Gly -10	-	expression tag
Ser -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Thr -2	-	expression tag
Asp -1	-	expression tag
Pro 0	-	expression tag
Met 1	-	expression tag
Val 2	-	expression tag
Leu 65	Phe 64	engineered mutation
Pia 68	Ser 65	chromophore
Pia 68	Tyr 66	chromophore
Pia 68	Gly 67	chromophore
Leu 70	Gln 69	engineered mutation
Ser 164	Val 163	engineered mutation
Ala 204	Thr 203	engineered mutation
Lys 207	Ala 206	engineered mutation
Leu 232	His 231	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	28502.2
Non-Polymers*	Number of molecules	9
	Total formula weight	840.8
All*	Total formula weight	29342.9

*Water molecules are not included.