7AM7
Crystal structure of Peptiligase mutant - M222P/L217H/A225N/F189W/N218D
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, C | Subtilisin BPN' | polymer | 272 | 27551.5 | 2 | UniProt (P00782) Pfam (PF00082) In PDB | Bacillus amyloliquefaciens | Alkaline protease,Subtilisin DFE,Subtilisin Novo |
2 | B | Subtilisin BPN' | polymer | 272 | 27535.5 | 1 | UniProt (P00782) Pfam (PF00082) In PDB | Bacillus amyloliquefaciens | Alkaline protease,Subtilisin DFE,Subtilisin Novo |
3 | P | Eglin C fragment | polymer | 13 | 1460.7 | 1 | UniProt (by SIFTS) (P01051) In PDB | Hirudo medicinalis | |
4 | A, B, C | GLYCEROL | non-polymer | 92.1 | 9 | Chemie (GOL) | |||
5 | A, B, C | SULFATE ION | non-polymer | 96.1 | 5 | Chemie (SO4) | |||
6 | C | TRIETHYLENE GLYCOL | non-polymer | 150.2 | 1 | Chemie (PGE) | |||
7 | water | water | 18.0 | 143 | Chemie (HOH) |
Sequence modifications
A, C: 1 - 275 (UniProt: P00782)
B: 1 - 275 (UniProt: P00782)
PDB | External Database | Details |
---|---|---|
Lys 2 | Gln 109 | engineered mutation |
Cys 3 | Ser 110 | engineered mutation |
Ser 5 | Pro 112 | engineered mutation |
Asn 43 | Lys 150 | engineered mutation |
Phe 50 | Met 157 | engineered mutation |
- | Ala 180 | deletion |
- | Ala 181 | deletion |
- | Leu 182 | deletion |
- | Asn 183 | deletion |
- | Asn 184 | deletion |
- | Ser 185 | deletion |
- | Ile 186 | deletion |
- | Gly 187 | deletion |
- | Val 188 | deletion |
Ala 74 | Gly 190 | engineered mutation |
Ser 156 | Glu 263 | engineered mutation |
Ser 166 | Gly 273 | engineered mutation |
Ala 169 | Gly 276 | engineered mutation |
Pro 188 | Ser 295 | engineered mutation |
Trp 189 | Phe 296 | engineered mutation |
Cys 206 | Gln 313 | engineered mutation |
His 217 | Tyr 324 | engineered mutation |
Asp 218 | Asn 325 | engineered mutation |
Cso 221 | Ser 328 | engineered mutation |
Pro 222 | Met 329 | engineered mutation |
Asn 225 | Pro 332 | engineered mutation |
Ala 254 | Thr 361 | engineered mutation |
Glu 271 | Gln 378 | engineered mutation |
His 276 | - | expression tag |
His 277 | - | expression tag |
His 278 | - | expression tag |
His 279 | - | expression tag |
His 280 | - | expression tag |
His 281 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Lys 2 | Gln 109 | engineered mutation |
Cys 3 | Ser 110 | engineered mutation |
Ser 5 | Pro 112 | engineered mutation |
Asn 43 | Lys 150 | engineered mutation |
Phe 50 | Met 157 | engineered mutation |
- | Ala 180 | deletion |
- | Ala 181 | deletion |
- | Leu 182 | deletion |
- | Asn 183 | deletion |
- | Asn 184 | deletion |
- | Ser 185 | deletion |
- | Ile 186 | deletion |
- | Gly 187 | deletion |
- | Val 188 | deletion |
Ala 74 | Gly 190 | engineered mutation |
Ser 156 | Glu 263 | engineered mutation |
Ser 166 | Gly 273 | engineered mutation |
Ala 169 | Gly 276 | engineered mutation |
Pro 188 | Ser 295 | engineered mutation |
Trp 189 | Phe 296 | engineered mutation |
Cys 206 | Gln 313 | engineered mutation |
His 217 | Tyr 324 | engineered mutation |
Asp 218 | Asn 325 | engineered mutation |
Cys 221 | Ser 328 | engineered mutation |
Pro 222 | Met 329 | engineered mutation |
Asn 225 | Pro 332 | engineered mutation |
Ala 254 | Thr 361 | engineered mutation |
Glu 271 | Gln 378 | engineered mutation |
His 276 | - | expression tag |
His 277 | - | expression tag |
His 278 | - | expression tag |
His 279 | - | expression tag |
His 280 | - | expression tag |
His 281 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 84099.0 | |
Non-Polymers* | Number of molecules | 15 |
Total formula weight | 1459.3 | |
All* | Total formula weight | 85558.4 |