7AM5
Crystal structure of Peptiligase mutant - L217H/M222P/A225N
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Subtilisin BPN' | polymer | 272 | 27468.4 | 1 | UniProt (P00782) Pfam (PF00082) In PDB | Bacillus amyloliquefaciens | Alkaline protease,Subtilisin DFE,Subtilisin Novo |
2 | A | SODIUM ION | non-polymer | 23.0 | 2 | Chemie (NA) | |||
3 | water | water | 18.0 | 102 | Chemie (HOH) |
Sequence modifications
A: 1 - 275 (UniProt: P00782)
PDB | External Database | Details |
---|---|---|
Lys 2 | Gln 109 | engineered mutation |
Cys 3 | Ser 110 | engineered mutation |
Ser 5 | Pro 112 | engineered mutation |
Asn 43 | Lys 150 | engineered mutation |
Phe 50 | Met 157 | engineered mutation |
- | Ala 180 | deletion |
- | Ala 181 | deletion |
- | Leu 182 | deletion |
- | Asn 183 | deletion |
- | Asn 184 | deletion |
- | Ser 185 | deletion |
- | Ile 186 | deletion |
- | Gly 187 | deletion |
- | Val 188 | deletion |
Ala 74 | Gly 190 | engineered mutation |
Ser 156 | Glu 263 | engineered mutation |
Ser 166 | Gly 273 | engineered mutation |
Ala 169 | Gly 276 | engineered mutation |
Pro 188 | Ser 295 | engineered mutation |
Cys 206 | Gln 313 | engineered mutation |
His 217 | Tyr 324 | engineered mutation |
Ser 218 | Asn 325 | engineered mutation |
Cys 221 | Ser 328 | engineered mutation |
Pro 222 | Met 329 | engineered mutation |
Asn 225 | Pro 332 | engineered mutation |
Ala 254 | Thr 361 | engineered mutation |
Glu 271 | Gln 378 | engineered mutation |
His 276 | - | expression tag |
His 277 | - | expression tag |
His 278 | - | expression tag |
His 279 | - | expression tag |
His 280 | - | expression tag |
His 281 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 27468.4 | |
Non-Polymers* | Number of molecules | 2 |
Total formula weight | 46.0 | |
All* | Total formula weight | 27514.4 |