7ALL

A single sulfatase is required for metabolism of colonic mucin O-glycans and intestinal colonization by a symbiotic human gut bacterium (BT4683-S1_4)

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (AAA)	Arylsulfatase	polymer	523	59468.3	1	UniProt (Q89YP8) Pfam (PF00884) Pfam (PF01663) Pfam (PF16347)	Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
2	B (A)	beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-alpha-D-glucopyranose	branched		383.3	1	In PDB BIRD (PRD_900019) GlyTouCan (G79333MY)
3	C (AAA)	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
4	D, E, F, G (AAA)	IODIDE ION	non-polymer		126.9	4	Chemie (IOD)
5	H (AAA)	water	water		18.0	309	Chemie (HOH)

Sequence modifications

AAA: 24 - 523 (UniProt: Q89YP8)

PDB	External Database	Details
Met 1	-	initiating methionine
Gly 2	-	expression tag
Ser 3	-	expression tag
Ser 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
Ser 11	-	expression tag
Ser 12	-	expression tag
Gly 13	-	expression tag
Leu 14	-	expression tag
Val 15	-	expression tag
Pro 16	-	expression tag
Arg 17	-	expression tag
Gly 18	-	expression tag
Ser 19	-	expression tag
His 20	-	expression tag
Met 21	-	expression tag
Ala 22	-	expression tag
Ser 23	-	expression tag
Cys 73	Ser 73	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	59468.3
Branched	Number of molecules	1
	Total formula weight	383.3
Non-Polymers*	Number of molecules	5
	Total formula weight	547.7
All*	Total formula weight	60399.3

*Water molecules are not included.