7AKQ
Structure of D169A/E171A double mutant of chitinase Chit42 from Trichoderma harzianum complexed with chitintetraose obtained by soaking.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | Endochitinase 42 | polymer | 423 | 45968.2 | 1 | UniProt (P48827) Pfam (PF00704) In PDB | Trichoderma harzianum | 42 kDa endochitinase,Chitinase 42 |
| 2 | B | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 1 | In PDB GlyTouCan (G42666HT) | |||
| 3 | C | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 830.8 | 1 | In PDB GlyTouCan (G01361SX) | |||
| 4 | A | ACETATE ION | non-polymer | 59.0 | 1 | Chemie (ACT) | |||
| 5 | A | ZINC ION | non-polymer | 65.4 | 5 | Chemie (ZN) | |||
| 6 | water | water | 18.0 | 207 | Chemie (HOH) |
Sequence modifications
A: 1 - 423 (UniProt: P48827)
| PDB | External Database | Details |
|---|---|---|
| Ala 169 | Asp 169 | engineered mutation |
| Ala 171 | Glu 171 | engineered mutation |
| Leu 193 | Arg 193 | engineered mutation |
| Leu 390 | Val 390 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 45968.2 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 1255.2 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 386.1 | |
| All* | Total formula weight | 47609.5 |
