7AKO

Crystal structure of CHK1 kinase domain in complex with a CLASPIN phosphopeptide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Serine/threonine-protein kinase Chk1	polymer	300	34357.4	2	UniProt (O14757) Pfam (PF00069)	Homo sapiens (Human)	CHK1 checkpoint homolog,Cell cycle checkpoint kinase,Checkpoint kinase-1
2	C, D (C, D)	Claspin	polymer	16	1896.0	2	UniProt (Q9HAW4)	Homo sapiens	hClaspin
3	E, I (A, B)	STAUROSPORINE	non-polymer		466.5	2	Chemie (STU)
4	F, G, H, J, K... (A, B)	1,2-ETHANEDIOL	non-polymer		62.1	7	Chemie (EDO)
5	N (C)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
6	O, P, Q, R (A, B, C, D)	water	water		18.0	314	Chemie (HOH)

Sequence modifications

A, B: 2 - 289 (UniProt: O14757)

PDB	External Database	Details
Gly -2	-	expression tag
Pro -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag
Arg 10	Asp 10	engineered mutation
His 290	-	expression tag
His 291	-	expression tag
His 292	-	expression tag
His 293	-	expression tag
His 294	-	expression tag
His 295	-	expression tag
His 296	-	expression tag
His 297	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	72506.7
Non-Polymers*	Number of molecules	10
	Total formula weight	1432.9
All*	Total formula weight	73939.7

*Water molecules are not included.