7AIC
MutS-MutL in clamp state (kinked clamp domain)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B | DNA mismatch repair protein MutS | polymer | 853 | 95269.6 | 2 | UniProt (P23909) Pfam (PF01624) Pfam (PF05188) Pfam (PF05192) Pfam (PF05190) Pfam (PF00488) In PDB | Escherichia coli (strain K12) | |
| 2 | C | DNA mismatch repair protein MutL | polymer | 351 | 39248.5 | 1 | UniProt (P23367) Pfam (PF13589) Pfam (PF01119) In PDB | Escherichia coli (strain K12) | |
| 3 | D | DNA (30-MER) | polymer | 30 | 9304.0 | 1 | synthetic construct | ||
| 4 | E | DNA (30-MER) | polymer | 30 | 9143.9 | 1 | synthetic construct | ||
| 5 | A, B | PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER | non-polymer | 506.2 | 2 | Chemie (ANP) |
Sequence modifications
A, B: 1 - 853 (UniProt: P23909)
C: 1 - 331 (UniProt: P23367)
| PDB | External Database | Details |
|---|---|---|
| Ala 93 | Cys 93 | engineered mutation |
| Ser 235 | Cys 235 | engineered mutation |
| Ala 239 | Cys 239 | engineered mutation |
| Cys 246 | Asp 246 | engineered mutation |
| Ser 297 | Cys 297 | engineered mutation |
| Ser 569 | Cys 569 | engineered mutation |
| Val 711 | Cys 711 | engineered mutation |
| Arg 835 | Asp 835 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -19 | - | initiating methionine |
| Gly -18 | - | expression tag |
| Ser -17 | - | expression tag |
| Ser -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| Ser -9 | - | expression tag |
| Ser -8 | - | expression tag |
| Gly -7 | - | expression tag |
| Leu -6 | - | expression tag |
| Val -5 | - | expression tag |
| Pro -4 | - | expression tag |
| Arg -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| His 0 | - | expression tag |
| Ser 61 | Cys 61 | engineered mutation |
| Cys 131 | Asn 131 | engineered mutation |
| Leu 216 | Cys 216 | engineered mutation |
| Phe 256 | Cys 256 | engineered mutation |
| Tyr 276 | Cys 276 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 248235.7 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 1012.4 | |
| All* | Total formula weight | 249248.1 |
