7AB7

Structure of Chloroflexus aggregans flavin based fluorescent protein (CagFbFP) I52T Q148K variant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Multi-sensor hybrid histidine kinase	polymer	113	12389.9	2	UniProt (B8GAY9) Pfam (PF13426)	Chloroflexus aggregans (strain MD-66 / DSM 9485)
2	C, E (A, B)	FLAVIN MONONUCLEOTIDE	non-polymer		456.3	2	Chemie (FMN)
3	D, G (A, B)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
4	F (B)	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
5	H, I (A, B)	water	water		18.0	175	Chemie (HOH)

Sequence modifications

A, B: 47 - 153 (UniProt: B8GAY9)

PDB	External Database	Details
Thr 52	Ile 52	engineered mutation
Ala 85	Cys 85	engineered mutation
Lys 148	Gln 148	engineered mutation
His 154	-	expression tag
His 155	-	expression tag
His 156	-	expression tag
His 157	-	expression tag
His 158	-	expression tag
His 159	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	24779.8
Non-Polymers*	Number of molecules	5
	Total formula weight	1192.9
All*	Total formula weight	25972.7

*Water molecules are not included.