7A9J

Crystal structure of the R395G mutant form of Coronafacic Acid Ligase from Pectobacterium brasiliense

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Cfl	polymer	537	60306.3	1	UniProt (M4GWN4) Pfam (PF00501) Pfam (PF13193) In PDB	Pectobacterium brasiliense	Ligase
2	A	6-ethyl-1-oxidanylidene-indene-4-carboxylic acid	non-polymer		202.2	1	Chemie (R4Z)
3	A	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
4		water	water		18.0	322	Chemie (HOH)

Sequence modifications

A: 1 - 516 (UniProt: M4GWN4)

PDB	External Database	Details
Met -20	-	initiating methionine
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Val -6	-	expression tag
Pro -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag
Gly 395	Arg 395	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	60306.3
Non-Polymers*	Number of molecules	3
	Total formula weight	392.1
All*	Total formula weight	60698.5

*Water molecules are not included.