7A86

rsGreen0.7-K206A-F145L partially in the green-off state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Green fluorescent protein	polymer	272	30577.3	2	UniProt (A0A059PIQ0) Pfam (PF01353) In PDB	Aequorea victoria (Jellyfish)
2	B	TRIETHYLENE GLYCOL	non-polymer		150.2	1	Chemie (PGE)
3		water	water		18.0	257	Chemie (HOH)

Sequence modifications

A, B: 3 - 238 (UniProt: A0A059PIQ0)

PDB	External Database	Details
Met -33	-	initiating methionine
Arg -32	-	expression tag
Gly -31	-	expression tag
Ser -30	-	expression tag
His -29	-	expression tag
His -28	-	expression tag
His -27	-	expression tag
His -26	-	expression tag
His -25	-	expression tag
His -24	-	expression tag
Gly -23	-	expression tag
Met -22	-	expression tag
Ala -21	-	expression tag
Ser -20	-	expression tag
Met -19	-	expression tag
Thr -18	-	expression tag
Gly -17	-	expression tag
Gly -16	-	expression tag
Gln -15	-	expression tag
Gln -14	-	expression tag
Met -13	-	expression tag
Gly -12	-	expression tag
Arg -11	-	expression tag
Asp -10	-	expression tag
Leu -9	-	expression tag
Tyr -8	-	expression tag
Asp -7	-	expression tag
Asp -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Lys -3	-	expression tag
Asp -2	-	expression tag
Pro -1	-	expression tag
Met 0	-	expression tag
Val 1	-	expression tag
Ser 2	-	expression tag
Arg 30	Ser 30	conflict
Cro 66	Thr 65	chromophore
Cro 66	Tyr 66	chromophore
Cro 66	Gly 67	chromophore
Leu 69	Gln 69	conflict
Tyr 105	Thr 105	conflict
Leu 145	Phe 145	engineered mutation
Ala 150	Val 150	conflict
Ser 163	Ala 163	conflict
Asn 205	Ser 205	conflict
Leu 231	His 231	conflict

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	61154.6
Non-Polymers*	Number of molecules	1
	Total formula weight	150.2
All*	Total formula weight	61304.8

*Water molecules are not included.