7A6D
tRNA-guanine transglycosylase C158S/C281S/Y330C/H333A mutant in complex with rac-trans-4,4-difluorocyclopentane-1,2-diol
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | Queuine tRNA-ribosyltransferase | polymer | 388 | 42910.6 | 1 | UniProt (P28720) Pfam (PF01702) In PDB | Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4) | Guanine insertion enzyme,tRNA-guanine transglycosylase |
| 2 | A | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 3 | A | GLYCEROL | non-polymer | 92.1 | 3 | Chemie (GOL) | |||
| 4 | A | 1,2-ETHANEDIOL | non-polymer | 62.1 | 2 | Chemie (EDO) | |||
| 5 | A | trans-4,4-difluorocyclopentane-1,2-diol | non-polymer | 138.1 | 1 | Chemie (R1E) | |||
| 6 | A | (1~{R},2~{R})-4,4-bis(fluoranyl)cyclopentane-1,2-diol | non-polymer | 138.1 | 1 | Chemie (RGN) | |||
| 7 | water | water | 18.0 | 261 | Chemie (HOH) |
Sequence modifications
A: 1 - 386 (UniProt: P28720)
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Ser 158 | Cys 158 | engineered mutation |
| Ser 281 | Cys 281 | engineered mutation |
| Lys 312 | Thr 312 | engineered mutation |
| Cys 330 | Tyr 330 | engineered mutation |
| Ala 333 | His 333 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 42910.6 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 742.1 | |
| All* | Total formula weight | 43652.7 |
