7A4N

Cryo-EM structure of a prefusion stabilized SARS-CoV-2 Spike (D614N, R682S, R685G, A892P, A942P and V987P)(S-closed trimer)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	Spike glycoprotein,Fibritin	polymer	1297	142547.3	3	UniProt (P0DTC2) UniProt (P10104) Pfam (PF16451) Pfam (PF09408) Pfam (PF19209) Pfam (PF01601)	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)	S glycoprotein,E2,Peplomer protein,Collar protein,Whisker antigen control protein
2	D, E, F (D, E, F)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	3	In PDB GlyTouCan (G42666HT)
3	G, H, I, J, K... (A, B, C)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	15	Chemie (NAG)

Sequence modifications

A, B, C: 1 - 1208 (UniProt: P0DTC2)

PDB	External Database	Details
Asn 614	Asp 614	engineered mutation
Ser 682	Arg 682	engineered mutation
Gly 685	Arg 685	engineered mutation
Pro 892	Ala 892	engineered mutation
Pro 942	Ala 942	engineered mutation
Pro 987	Val 987	engineered mutation
Gly 1209	-	linker
Ser 1210	-	linker

A, B, C: 1211 - 1237 (UniProt: P10104)

PDB	External Database	Details
Leu 1232	Phe 479	conflict
Gly 1238	-	expression tag
Arg 1239	-	expression tag
Ser 1240	-	expression tag
Leu 1241	-	expression tag
Glu 1242	-	expression tag
Val 1243	-	expression tag
Leu 1244	-	expression tag
Phe 1245	-	expression tag
Gln 1246	-	expression tag
Gly 1247	-	expression tag
Pro 1248	-	expression tag
Gly 1249	-	expression tag
Ser 1250	-	expression tag
Leu 1251	-	expression tag
Pro 1252	-	expression tag
Glu 1253	-	expression tag
Thr 1254	-	expression tag
Gly 1255	-	expression tag
Gly 1256	-	expression tag
Gly 1257	-	expression tag
Ser 1258	-	expression tag
Asp 1259	-	expression tag
Tyr 1260	-	expression tag
Lys 1261	-	expression tag
Asp 1262	-	expression tag
Asp 1263	-	expression tag
Asp 1264	-	expression tag
Asp 1265	-	expression tag
Lys 1266	-	expression tag
Gly 1267	-	expression tag
Gly 1268	-	expression tag
Gly 1269	-	expression tag
Gly 1270	-	expression tag
Ser 1271	-	expression tag
Gly 1272	-	expression tag
Gly 1273	-	expression tag
Gly 1274	-	expression tag
Gly 1275	-	expression tag
Ser 1276	-	expression tag
Gly 1277	-	expression tag
Gly 1278	-	expression tag
Gly 1279	-	expression tag
Gly 1280	-	expression tag
Ser 1281	-	expression tag
Gly 1282	-	expression tag
Gly 1283	-	expression tag
Gly 1284	-	expression tag
Gly 1285	-	expression tag
Ser 1286	-	expression tag
Gly 1287	-	expression tag
Gly 1288	-	expression tag
Gly 1289	-	expression tag
Gly 1290	-	expression tag
Ser 1291	-	expression tag
His 1292	-	expression tag
His 1293	-	expression tag
His 1294	-	expression tag
His 1295	-	expression tag
His 1296	-	expression tag
His 1297	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	427642.0
Branched	Number of molecules	3
	Total formula weight	1273.2
Non-Polymers*	Number of molecules	15
	Total formula weight	3318.1
All*	Total formula weight	432233.4

*Water molecules are not included.