7A3V
tRNA-guanine transglycosylase C158S/C281S/Y330C/H333A mutant in complex with 3-hydroxysulfolane
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Queuine tRNA-ribosyltransferase | polymer | 388 | 42910.6 | 1 | UniProt (P28720) Pfam (PF01702) In PDB | Zymomonas mobilis subsp. mobilis ZM4 = ATCC 31821 | Guanine insertion enzyme,tRNA-guanine transglycosylase |
2 | A | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
3 | A | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
4 | A | 3-hydroxythietane-1,1-dioxide | non-polymer | 136.2 | 2 | Chemie (QXE) | |||
5 | A | 1,2-ETHANEDIOL | non-polymer | 62.1 | 1 | Chemie (EDO) | |||
6 | water | water | 18.0 | 211 | Chemie (HOH) |
Sequence modifications
A: 1 - 386 (UniProt: P28720)
PDB | External Database | Details |
---|---|---|
Gly -1 | - | expression tag |
Ser 0 | - | expression tag |
Ser 158 | Cys 158 | engineered mutation |
Ser 281 | Cys 281 | engineered mutation |
Lys 312 | Thr 312 | engineered mutation |
Cys 330 | Tyr 330 | engineered mutation |
Ala 333 | His 333 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 42910.6 | |
Non-Polymers* | Number of molecules | 6 |
Total formula weight | 584.0 | |
All* | Total formula weight | 43494.6 |