7A0U

LIGAND FREE TYPE II E. COLI ASPARAGINASE T12S/T89S DOUBLE MUTANT

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	L-asparaginase 2	polymer	333	35558.8	4	UniProt (P00805) Pfam (PF00710) Pfam (PF17763)	Escherichia coli (strain K12)	L-asparaginase II,L-ASNase II,L-asparagine amidohydrolase II
2	E, F, G, H (A, B, C, D)	water	water		18.0	680	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 326 (UniProt: P00805)

PDB	External Database	Details
Met -6	-	initiating methionine
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Ser 12	Thr 34	engineered mutation
Ser 89	Thr 111	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	142235.3
All*	Total formula weight	142235.3

*Water molecules are not included.