7A0B
tRNA-guanine transglycosylase C158S/C281S/Y330C/H333A mutant in complex with rac-trans-3,4-dihydroxysulfolane
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Queuine tRNA-ribosyltransferase | polymer | 388 | 42910.6 | 1 | UniProt (P28720) Pfam (PF01702) | Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4) | Guanine insertion enzyme,tRNA-guanine transglycosylase |
| 2 | B (A) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 3 | C, D, E, F (A) | GLYCEROL | non-polymer | 92.1 | 4 | Chemie (GOL) | |||
| 4 | G, H (A) | (3~{R},4~{R})-1,1-bis(oxidanylidene)thiolane-3,4-diol | non-polymer | 152.2 | 2 | Chemie (QTQ) | |||
| 5 | I (A) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 1 | Chemie (EDO) | |||
| 6 | J (A) | water | water | 18.0 | 182 | Chemie (HOH) |
Sequence modifications
A: 1 - 386 (UniProt: P28720)
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Ser 158 | Cys 158 | engineered mutation |
| Ser 281 | Cys 281 | engineered mutation |
| Lys 312 | Thr 312 | engineered mutation |
| Cys 330 | Tyr 330 | engineered mutation |
| Ala 333 | His 333 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 42910.6 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 800.2 | |
| All* | Total formula weight | 43710.8 |
