6ZY9

Cryo-EM structure of MlaFEDB in complex with AMP-PNP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D, I, J, K...	YrbD protein	polymer	183	19593.1	6	UniProt (D6IEA5) Pfam (PF02470) UniProt (by SIFTS) (P64604) In PDB	Escherichia coli B185
2	B, C	ABC transporter maintaining OM lipid asymmetry, cytoplasmic STAS component	polymer	105	11795.5	2	UniProt (W8T4U6) Pfam (PF13466) UniProt (by SIFTS) (P64602) In PDB	Escherichia coli	Lipid asymmetry maintenance protein MlaB,Phospholipid ABC transporter substrate-binding protein,Phospholipid ABC transporter-binding protein,Putative anti-sigma factor antagonist,Putative phospholipid ABC transporter-binding protein MlaB
3	F, G	Toluene tolerance protein Ttg2A	polymer	269	29128.8	2	UniProt (V0AC37) In PDB	Escherichia coli 909945-2
4	E, H	Uncharacterized protein	polymer	260	27885.2	2	UniProt (I2X585) In PDB	Escherichia coli 2.3916
5	F, G	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	2	Chemie (ANP)
6	F, G	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Sequence modifications

B, C: 1 - 97 (UniProt: W8T4U6)

PDB	External Database	Details
His 98	-	expression tag
His 99	-	expression tag
His 100	-	expression tag
His 101	-	expression tag
His 102	-	expression tag
His 103	-	expression tag
His 104	-	expression tag
His 105	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	255177.7
Non-Polymers*	Number of molecules	4
	Total formula weight	1061.0
All*	Total formula weight	256238.7

*Water molecules are not included.