6ZTO

E. coli 70S-RNAP expressome complex in uncoupled state 1

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (AA)	16S ribosomal RNA	polymer	1542	499888.4	1	GenBank (CP054232.1)	Escherichia coli
2	B (AB)	30S ribosomal protein S2	polymer	241	26781.7	1	UniProt (C3TPN2) Pfam (PF00318) UniProt (by SIFTS) (P0A7V0)	Escherichia coli
3	C (AC)	30S ribosomal protein S3	polymer	233	26031.3	1	UniProt (C3SQX2) Pfam (PF07650) Pfam (PF00189) UniProt (by SIFTS) (P0A7V3)	Escherichia coli
4	D (AD)	30S ribosomal protein S4	polymer	206	23514.2	1	UniProt (C3SR62) Pfam (PF00163) Pfam (PF01479) UniProt (by SIFTS) (P0A7V8)	Escherichia coli
5	E (AE)	30S ribosomal protein S5	polymer	167	17617.5	1	UniProt (A0A090BZW5) Pfam (PF00333) Pfam (PF03719) UniProt (by SIFTS) (P0A7W1)	Escherichia coli
6	F (AF)	30S ribosomal protein S6	polymer	131	15197.0	1	UniProt (W8T6F0) Pfam (PF01250) UniProt (by SIFTS) (P02358)	Escherichia coli
7	G (AG)	30S ribosomal protein S7	polymer	156	17637.4	1	UniProt (P02359) Pfam (PF00177)	Escherichia coli	Small ribosomal subunit protein uS7
8	H (AH)	30S ribosomal protein S8	polymer	130	14146.6	1	UniProt (C3SR12) Pfam (PF00410) UniProt (by SIFTS) (P0A7W7)	Escherichia coli
9	I (AI)	30S ribosomal protein S9	polymer	130	14886.3	1	UniProt (C3SRY2) Pfam (PF00380) UniProt (by SIFTS) (P0A7X3)	Escherichia coli
10	J (AJ)	30S ribosomal protein S10	polymer	103	11755.6	1	UniProt (C3SQT7) Pfam (PF00338) UniProt (by SIFTS) (P0A7R5)	Escherichia coli
11	K (AK)	30S ribosomal protein S11	polymer	129	13871.0	1	UniProt (C3SR57) Pfam (PF00411) UniProt (by SIFTS) (P0A7R9)	Escherichia coli
12	L (AL)	30S ribosomal protein S12	polymer	124	13814.2	1	UniProt (C3SQR7) Pfam (PF00164) UniProt (by SIFTS) (P0A7S3)	Escherichia coli
13	M (AM)	30S ribosomal protein S13	polymer	118	13128.5	1	UniProt (C3SR52) Pfam (PF00416) UniProt (by SIFTS) (P0A7S9)	Escherichia coli
14	N (AN)	30S ribosomal protein S14	polymer	101	11606.6	1	UniProt (C3SR07) Pfam (PF00253) UniProt (by SIFTS) (P0AG59)	Escherichia coli
15	O (AO)	30S ribosomal protein S15	polymer	89	10290.8	1	UniProt (C3SSQ7) Pfam (PF00312) UniProt (by SIFTS) (P0ADZ4)	Escherichia coli
16	P (AP)	30S ribosomal protein S16	polymer	82	9207.6	1	UniProt (C3SYP2) Pfam (PF00886) UniProt (by SIFTS) (P0A7T3)	Escherichia coli
17	Q (AQ)	30S ribosomal protein S17	polymer	84	9724.5	1	UniProt (C3SQY7) Pfam (PF00366) UniProt (by SIFTS) (P0AG63)	Escherichia coli
18	R (AR)	30S ribosomal protein S18	polymer	75	9005.5	1	UniProt (C3SFP7) Pfam (PF01084) UniProt (by SIFTS) (P0A7T7)	Escherichia coli
19	S (AS)	30S ribosomal protein S19	polymer	92	10455.4	1	UniProt (C3SQW2) Pfam (PF00203) UniProt (by SIFTS) (P0A7U3)	Escherichia coli
20	T (AT)	30S ribosomal protein S20	polymer	87	9708.5	1	UniProt (C3TRH7) Pfam (PF01649) UniProt (by SIFTS) (P0A7U7)	Escherichia coli
21	U (AU)	30S ribosomal protein S21	polymer	71	8524.0	1	UniProt (C3STZ7) Pfam (PF01165) UniProt (by SIFTS) (P68679)	Escherichia coli
22	V (AV)	mRNA	polymer	53	17060.3	1		synthetic construct
23	W (AW)	tRNA(fmet) P-site	polymer	77	24832.9	1		Escherichia coli
24	X (AX)	Phe-NH-tRNA(Phe) A-site	polymer	76	24751.0	1	GenBank (CP054236.1)	Escherichia coli
25	Y (BA)	23S ribosomal RNA	polymer	2904	941820.6	1		Escherichia coli
26	Z (BB)	5S ribosomal RNA	polymer	120	38790.1	1	GenBank (CP035706.1)	Escherichia coli
27	AA (BC)	50S ribosomal protein L2	polymer	273	29923.6	1	UniProt (P60422) Pfam (PF00181) Pfam (PF03947)	Escherichia coli	ribosomal protein uL2
28	BA (BD)	50S ribosomal protein L3	polymer	209	22291.6	1	UniProt (P60438) Pfam (PF00297)	Escherichia coli	Large ribosomal subunit protein uL3
29	CA (BE)	50S ribosomal protein L4	polymer	201	22121.6	1	UniProt (P60723) Pfam (PF00573)	Escherichia coli	ribosomal protein uL4
30	DA (BF)	50S ribosomal protein L5	polymer	179	20333.6	1	UniProt (P62399) Pfam (PF00281) Pfam (PF00673)	Escherichia coli	ribosomal protein uL5
31	EA (BG)	50S ribosomal protein L6	polymer	177	18932.8	1	UniProt (P0AG55) Pfam (PF00347)	Escherichia coli	ribosomal protein uL6
32	FA (BH)	50S ribosomal protein L9	polymer	149	15789.0	1	UniProt (P0A7R1) Pfam (PF01281) Pfam (PF03948)	Escherichia coli	ribosomal protein bL9
33	GA (BK)	50S ribosomal protein L13	polymer	142	16050.6	1	UniProt (P0AA10) Pfam (PF00572)	Escherichia coli	ribosomal protein uL13
34	HA (BL)	50S ribosomal protein L14	polymer	123	13565.1	1	UniProt (P0ADY3) Pfam (PF00238)	Escherichia coli	ribosomal protein uL14
35	IA (BM)	50S ribosomal protein L15	polymer	144	14994.4	1	UniProt (P02413) Pfam (PF00828)	Escherichia coli	Large ribosomal subunit protein uL15
36	JA (BN)	50S ribosomal protein L16	polymer	136	15327.3	1	UniProt (P0ADY7) Pfam (PF00252)	Escherichia coli	Large ribosomal subunit protein uL16
37	KA (BO)	50S ribosomal protein L17	polymer	127	14393.7	1	UniProt (P0AG44) Pfam (PF01196)	Escherichia coli	ribosomal protein bL17
38	LA (BP)	50S ribosomal protein L18	polymer	117	12794.7	1	UniProt (P0C018) Pfam (PF00861)	Escherichia coli	ribosomal protein uL18
39	MA (BQ)	50S ribosomal protein L19	polymer	115	13159.3	1	UniProt (P0A7K6) Pfam (PF01245)	Escherichia coli	ribosomal protein bL19
40	NA (BR)	50S ribosomal protein L20	polymer	118	13528.0	1	UniProt (P0A7L3) Pfam (PF00453)	Escherichia coli	ribosomal protein bL20
41	OA (BS)	50S ribosomal protein L21	polymer	103	11586.4	1	UniProt (P0AG48) Pfam (PF00829)	Escherichia coli	ribosomal protein bL21
42	PA (BT)	50S ribosomal protein L22	polymer	110	12253.4	1	UniProt (P61175) Pfam (PF00237)	Escherichia coli	ribosomal protein uL22
43	QA (BU)	50S ribosomal protein L23	polymer	100	11222.2	1	UniProt (P0ADZ0) Pfam (PF00276)	Escherichia coli	ribosomal protein uL23
44	RA (BV)	50S ribosomal protein L24	polymer	104	11339.3	1	UniProt (P60624) Pfam (PF00467) Pfam (PF17136)	Escherichia coli	ribosomal protein uL24
45	SA (BW)	50S ribosomal protein L25	polymer	94	10713.5	1	UniProt (P68919) Pfam (PF01386)	Escherichia coli	ribosomal protein bL25
46	TA (BX)	50S ribosomal protein L27	polymer	85	9146.5	1	UniProt (P0A7L8) Pfam (PF01016)	Escherichia coli	ribosomal protein bL27
47	UA (BY)	50S ribosomal protein L28	polymer	78	9027.6	1	UniProt (P0A7M2) Pfam (PF00830)	Escherichia coli	ribosomal protein bL28
48	VA (BZ)	50S ribosomal protein L29	polymer	63	7286.5	1	UniProt (P0A7M6) Pfam (PF00831)	Escherichia coli	ribosomal protein uL29
49	WA (B1)	50S ribosomal protein L30	polymer	59	6554.8	1	UniProt (P0AG51) Pfam (PF00327)	Escherichia coli	ribosomal protein uL30
50	XA (B2)	50S ribosomal protein L32	polymer	57	6463.4	1	UniProt (P0A7N4) Pfam (PF01783)	Escherichia coli	ribosomal protein bL32
51	YA (B3)	50S ribosomal protein L33	polymer	55	6388.6	1	UniProt (P0A7N9) Pfam (PF00471)	Escherichia coli	ribosomal protein bL33
52	ZA (B4)	50S ribosomal protein L34	polymer	46	5397.5	1	UniProt (P0A7P5) Pfam (PF00468)	Escherichia coli	ribosomal protein bL34
53	AB (B5)	50S ribosomal protein L35	polymer	65	7313.0	1	UniProt (P0A7Q1) Pfam (PF01632)	Escherichia coli	Ribosomal protein A, ribosomal protein bL35
54	BB (B6)	50S ribosomal protein L36	polymer	50	5720.1	1	UniProt (A0A2X1PTA3) Pfam (PF00444) UniProt (by SIFTS) (P0A7Q6)	Escherichia coli
55	CB (B7)	50S ribosomal protein L31	polymer	70	7887.1	1	UniProt (U9XX47) Pfam (PF01197) UniProt (by SIFTS) (P0A7M9)	Escherichia coli
56	DB (CN)	Non-template DNA strand	polymer	39	12063.8	1		synthetic construct
57	EB (CT)	Template DNA strand	polymer	39	11872.6	1		synthetic construct
58	FB, GB (CA, CB)	DNA-directed RNA polymerase subunit alpha	polymer	329	36558.7	2	UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118)	Escherichia coli	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
59	HB (CC)	DNA-directed RNA polymerase subunit beta	polymer	1342	150820.9	1	UniProt (P0A8V4) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) UniProt (by SIFTS) (P0A8V2)	Escherichia coli	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
60	IB (CD)	DNA-directed RNA polymerase subunit beta'	polymer	1407	155366.8	1	UniProt (P0A8T8) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) UniProt (by SIFTS) (P0A8T7)	Escherichia coli	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
61	JB (CE)	DNA-directed RNA polymerase subunit omega	polymer	91	10249.5	1	UniProt (P0A800) Pfam (PF01192)	Escherichia coli	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
62	AC, AD, AE, AF, AG... (AA, AW, BA, BQ, BV...)	MAGNESIUM ION	non-polymer		24.3	487	Chemie (MG)
63	FH (AX)	PHENYLALANINE	non-polymer		165.2	1	Chemie (PHE)
64	DU, EU, GU, HU (B6, B7, CD)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)

Sequence modifications

AE: 1 - 167 (UniProt: A0A090BZW5)

PDB	External Database	Details
Cys 9	Gly 9	conflict

BM: 1 - 144 (UniProt: P02413)

PDB	External Database	Details
Val 77	Ile 77	conflict

Contents of the asymmetric unit

Polymers	Number of chains	62
	Total formula weight	2619043.1
Non-Polymers*	Number of molecules	492
	Total formula weight	12263.4
All*	Total formula weight	2631306.4

*Water molecules are not included.