6ZTN
E. coli 70S-RNAP expressome complex in NusG-coupled state (42 nt intervening mRNA)
This is a non-PDB format compatible entry.
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | AA | 16S ribosomal RNA | polymer | 1542 | 499888.4 | 1 | GenBank (CP054232.1) | Escherichia coli | |
2 | AB | 30S ribosomal protein S2 | polymer | 241 | 26781.7 | 1 | UniProt (C3TPN2) Pfam (PF00318) UniProt (by SIFTS) (P0A7V0) In PDB | Escherichia coli | |
3 | AC | 30S ribosomal protein S3 | polymer | 233 | 26031.3 | 1 | UniProt (C3SQX2) Pfam (PF07650) Pfam (PF00189) UniProt (by SIFTS) (P0A7V3) In PDB | Escherichia coli | |
4 | AD | 30S ribosomal protein S4 | polymer | 206 | 23514.2 | 1 | UniProt (C3SR62) Pfam (PF00163) Pfam (PF01479) UniProt (by SIFTS) (P0A7V8) In PDB | Escherichia coli | |
5 | AE | 30S ribosomal protein S5 | polymer | 167 | 17617.5 | 1 | UniProt (A0A090BZW5) Pfam (PF00333) Pfam (PF03719) UniProt (by SIFTS) (P0A7W1) In PDB | Escherichia coli | |
6 | AF | 30S ribosomal protein S6 | polymer | 131 | 15197.0 | 1 | UniProt (W8T6F0) Pfam (PF01250) UniProt (by SIFTS) (P02358) In PDB | Escherichia coli | |
7 | AG | 30S ribosomal protein S7 | polymer | 156 | 17637.4 | 1 | UniProt (P02359) Pfam (PF00177) In PDB | Escherichia coli | Small ribosomal subunit protein uS7 |
8 | AH | 30S ribosomal protein S8 | polymer | 130 | 14146.6 | 1 | UniProt (C3SR12) Pfam (PF00410) UniProt (by SIFTS) (P0A7W7) In PDB | Escherichia coli | |
9 | AI | 30S ribosomal protein S9 | polymer | 130 | 14886.3 | 1 | UniProt (C3SRY2) Pfam (PF00380) UniProt (by SIFTS) (P0A7X3) In PDB | Escherichia coli | |
10 | AJ | 30S ribosomal protein S10 | polymer | 103 | 11755.6 | 1 | UniProt (C3SQT7) Pfam (PF00338) UniProt (by SIFTS) (P0A7R5) In PDB | Escherichia coli | |
11 | AK | 30S ribosomal protein S11 | polymer | 129 | 13871.0 | 1 | UniProt (C3SR57) Pfam (PF00411) UniProt (by SIFTS) (P0A7R9) In PDB | Escherichia coli | |
12 | AL | 30S ribosomal protein S12 | polymer | 124 | 13814.2 | 1 | UniProt (C3SQR7) Pfam (PF00164) UniProt (by SIFTS) (P0A7S3) In PDB | Escherichia coli | |
13 | AM | 30S ribosomal protein S13 | polymer | 118 | 13128.5 | 1 | UniProt (C3SR52) Pfam (PF00416) UniProt (by SIFTS) (P0A7S9) In PDB | Escherichia coli | |
14 | AN | 30S ribosomal protein S14 | polymer | 101 | 11606.6 | 1 | UniProt (C3SR07) Pfam (PF00253) UniProt (by SIFTS) (P0AG59) In PDB | Escherichia coli | |
15 | AO | 30S ribosomal protein S15 | polymer | 89 | 10290.8 | 1 | UniProt (C3SSQ7) Pfam (PF00312) UniProt (by SIFTS) (P0ADZ4) In PDB | Escherichia coli | |
16 | AP | 30S ribosomal protein S16 | polymer | 82 | 9207.6 | 1 | UniProt (C3SYP2) Pfam (PF00886) UniProt (by SIFTS) (P0A7T3) In PDB | Escherichia coli | |
17 | AQ | 30S ribosomal protein S17 | polymer | 84 | 9724.5 | 1 | UniProt (C3SQY7) Pfam (PF00366) UniProt (by SIFTS) (P0AG63) In PDB | Escherichia coli | |
18 | AR | 30S ribosomal protein S18 | polymer | 75 | 9005.5 | 1 | UniProt (C3SFP7) Pfam (PF01084) UniProt (by SIFTS) (P0A7T7) In PDB | Escherichia coli | |
19 | AS | 30S ribosomal protein S19 | polymer | 92 | 10455.4 | 1 | UniProt (C3SQW2) Pfam (PF00203) UniProt (by SIFTS) (P0A7U3) In PDB | Escherichia coli | |
20 | AT | 30S ribosomal protein S20 | polymer | 87 | 9708.5 | 1 | UniProt (C3TRH7) Pfam (PF01649) UniProt (by SIFTS) (P0A7U7) In PDB | Escherichia coli | |
21 | AU | 30S ribosomal protein S21 | polymer | 71 | 8524.0 | 1 | UniProt (C3STZ7) Pfam (PF01165) UniProt (by SIFTS) (P68679) In PDB | Escherichia coli | |
22 | AV | mRNA | polymer | 57 | 18409.1 | 1 | synthetic construct | ||
23 | AW | tRNA(fmet) P-site | polymer | 77 | 24832.9 | 1 | Escherichia coli | ||
24 | AX, AZ | Phe-NH-tRNA(Phe) A-site | polymer | 76 | 24751.0 | 2 | GenBank (CP054236.1) | Escherichia coli | |
25 | BA | 23S ribosomal RNA | polymer | 2904 | 941820.6 | 1 | Escherichia coli | ||
26 | BB | 5S ribosomal RNA | polymer | 120 | 38790.1 | 1 | GenBank (CP035706.1) | Escherichia coli | |
27 | BC | 50S ribosomal protein L2 | polymer | 273 | 29923.6 | 1 | UniProt (P60422) Pfam (PF00181) Pfam (PF03947) In PDB | Escherichia coli | ribosomal protein uL2 |
28 | BD | 50S ribosomal protein L3 | polymer | 209 | 22291.6 | 1 | UniProt (P60438) Pfam (PF00297) In PDB | Escherichia coli | Large ribosomal subunit protein uL3 |
29 | BE | 50S ribosomal protein L4 | polymer | 201 | 22121.6 | 1 | UniProt (P60723) Pfam (PF00573) In PDB | Escherichia coli | ribosomal protein uL4 |
30 | BF | 50S ribosomal protein L5 | polymer | 179 | 20333.6 | 1 | UniProt (P62399) Pfam (PF00281) Pfam (PF00673) In PDB | Escherichia coli | ribosomal protein uL5 |
31 | BG | 50S ribosomal protein L6 | polymer | 177 | 18932.8 | 1 | UniProt (P0AG55) Pfam (PF00347) In PDB | Escherichia coli | ribosomal protein uL6 |
32 | BH | 50S ribosomal protein L9 | polymer | 149 | 15789.0 | 1 | UniProt (P0A7R1) Pfam (PF01281) Pfam (PF03948) In PDB | Escherichia coli | ribosomal protein bL9 |
33 | BK | 50S ribosomal protein L13 | polymer | 142 | 16050.6 | 1 | UniProt (P0AA10) Pfam (PF00572) In PDB | Escherichia coli | ribosomal protein uL13 |
34 | BL | 50S ribosomal protein L14 | polymer | 123 | 13565.1 | 1 | UniProt (P0ADY3) Pfam (PF00238) In PDB | Escherichia coli | ribosomal protein uL14 |
35 | BM | 50S ribosomal protein L15 | polymer | 144 | 14994.4 | 1 | UniProt (P02413) Pfam (PF00828) In PDB | Escherichia coli | Large ribosomal subunit protein uL15 |
36 | BN | 50S ribosomal protein L16 | polymer | 136 | 15327.3 | 1 | UniProt (P0ADY7) Pfam (PF00252) In PDB | Escherichia coli | Large ribosomal subunit protein uL16 |
37 | BO | 50S ribosomal protein L17 | polymer | 127 | 14393.7 | 1 | UniProt (P0AG44) Pfam (PF01196) In PDB | Escherichia coli | ribosomal protein bL17 |
38 | BP | 50S ribosomal protein L18 | polymer | 117 | 12794.7 | 1 | UniProt (P0C018) Pfam (PF00861) In PDB | Escherichia coli | ribosomal protein uL18 |
39 | BQ | 50S ribosomal protein L19 | polymer | 115 | 13159.3 | 1 | UniProt (P0A7K6) Pfam (PF01245) In PDB | Escherichia coli | ribosomal protein bL19 |
40 | BR | 50S ribosomal protein L20 | polymer | 118 | 13528.0 | 1 | UniProt (P0A7L3) Pfam (PF00453) In PDB | Escherichia coli | ribosomal protein bL20 |
41 | BS | 50S ribosomal protein L21 | polymer | 103 | 11586.4 | 1 | UniProt (P0AG48) Pfam (PF00829) In PDB | Escherichia coli | ribosomal protein bL21 |
42 | BT | 50S ribosomal protein L22 | polymer | 110 | 12253.4 | 1 | UniProt (P61175) Pfam (PF00237) In PDB | Escherichia coli | ribosomal protein uL22 |
43 | BU | 50S ribosomal protein L23 | polymer | 100 | 11222.2 | 1 | UniProt (P0ADZ0) Pfam (PF00276) In PDB | Escherichia coli | ribosomal protein uL23 |
44 | BV | 50S ribosomal protein L24 | polymer | 104 | 11339.3 | 1 | UniProt (P60624) Pfam (PF00467) Pfam (PF17136) In PDB | Escherichia coli | ribosomal protein uL24 |
45 | BW | 50S ribosomal protein L25 | polymer | 94 | 10713.5 | 1 | UniProt (P68919) Pfam (PF01386) In PDB | Escherichia coli | ribosomal protein bL25 |
46 | BX | 50S ribosomal protein L27 | polymer | 85 | 9146.5 | 1 | UniProt (P0A7L8) Pfam (PF01016) In PDB | Escherichia coli | ribosomal protein bL27 |
47 | BY | 50S ribosomal protein L28 | polymer | 78 | 9027.6 | 1 | UniProt (P0A7M2) Pfam (PF00830) In PDB | Escherichia coli | ribosomal protein bL28 |
48 | BZ | 50S ribosomal protein L29 | polymer | 63 | 7286.5 | 1 | UniProt (P0A7M6) Pfam (PF00831) In PDB | Escherichia coli | ribosomal protein uL29 |
49 | B1 | 50S ribosomal protein L30 | polymer | 59 | 6554.8 | 1 | UniProt (P0AG51) Pfam (PF00327) In PDB | Escherichia coli | ribosomal protein uL30 |
50 | B2 | 50S ribosomal protein L32 | polymer | 57 | 6463.4 | 1 | UniProt (P0A7N4) Pfam (PF01783) In PDB | Escherichia coli | ribosomal protein bL32 |
51 | B3 | 50S ribosomal protein L33 | polymer | 55 | 6388.6 | 1 | UniProt (P0A7N9) Pfam (PF00471) In PDB | Escherichia coli | ribosomal protein bL33 |
52 | B4 | 50S ribosomal protein L34 | polymer | 46 | 5397.5 | 1 | UniProt (P0A7P5) Pfam (PF00468) In PDB | Escherichia coli | ribosomal protein bL34 |
53 | B5 | 50S ribosomal protein L35 | polymer | 65 | 7313.0 | 1 | UniProt (P0A7Q1) Pfam (PF01632) In PDB | Escherichia coli | Ribosomal protein A, ribosomal protein bL35 |
54 | B6 | 50S ribosomal protein L36 | polymer | 50 | 5720.1 | 1 | UniProt (A0A2X1PTA3) Pfam (PF00444) UniProt (by SIFTS) (P0A7Q6) In PDB | Escherichia coli | |
55 | CA, CB | DNA-directed RNA polymerase subunit alpha | polymer | 329 | 36558.7 | 2 | UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) In PDB | Escherichia coli | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
56 | CC | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 1 | UniProt (P0A8V4) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) UniProt (by SIFTS) (P0A8V2) In PDB | Escherichia coli | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
57 | CD | DNA-directed RNA polymerase subunit beta' | polymer | 1407 | 155366.8 | 1 | UniProt (P0A8T8) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) UniProt (by SIFTS) (P0A8T7) In PDB | Escherichia coli | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
58 | CE | DNA-directed RNA polymerase subunit omega | polymer | 91 | 10249.5 | 1 | UniProt (P0A800) Pfam (PF01192) In PDB | Escherichia coli | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
59 | CN | Non-template DNA strand | polymer | 39 | 12063.8 | 1 | synthetic construct | ||
60 | CT | Template DNA strand | polymer | 39 | 11872.6 | 1 | synthetic construct | ||
61 | CF | Transcription termination/antitermination protein NusG | polymer | 181 | 20560.5 | 1 | UniProt (C3SID2) Pfam (PF02357) Pfam (PF00467) UniProt (by SIFTS) (P0AFG0) In PDB | Escherichia coli | |
62 | AA, AW, BA, BX, B2... | MAGNESIUM ION | non-polymer | 24.3 | 487 | Chemie (MG) | |||
63 | AX | PHENYLALANINE | non-polymer | 165.2 | 1 | Chemie (PHE) | |||
64 | CD | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) |
Sequence modifications
AE: 1 - 167 (UniProt: A0A090BZW5)
BM: 1 - 144 (UniProt: P02413)
PDB | External Database | Details |
---|---|---|
Cys 9 | Gly 9 | conflict |
PDB | External Database | Details |
---|---|---|
Val 77 | Ile 77 | conflict |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 63 |
Total formula weight | 2657816.3 | |
Non-Polymers* | Number of molecules | 490 |
Total formula weight | 12132.5 | |
All* | Total formula weight | 2669948.9 |