6ZTJ
E. coli 70S-RNAP expressome complex in NusG-coupled state (38 nt intervening mRNA)
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (AA) | 16S ribosomal RNA | polymer | 1542 | 499888.4 | 1 | GenBank (CP054232.1) | Escherichia coli | |
| 2 | B (AB) | 30S ribosomal protein S2 | polymer | 241 | 26781.7 | 1 | UniProt (C3TPN2) Pfam (PF00318) UniProt (by SIFTS) (P0A7V0) | Escherichia coli | |
| 3 | C (AC) | 30S ribosomal protein S3 | polymer | 233 | 26031.3 | 1 | UniProt (C3SQX2) Pfam (PF07650) Pfam (PF00189) UniProt (by SIFTS) (P0A7V3) | Escherichia coli | |
| 4 | D (AD) | 30S ribosomal protein S4 | polymer | 206 | 23514.2 | 1 | UniProt (C3SR62) Pfam (PF00163) Pfam (PF01479) UniProt (by SIFTS) (P0A7V8) | Escherichia coli | |
| 5 | E (AE) | 30S ribosomal protein S5 | polymer | 167 | 17617.5 | 1 | UniProt (A0A090BZW5) Pfam (PF00333) Pfam (PF03719) UniProt (by SIFTS) (P0A7W1) | Escherichia coli | |
| 6 | F (AF) | 30S ribosomal protein S6 | polymer | 131 | 15197.0 | 1 | UniProt (W8T6F0) Pfam (PF01250) UniProt (by SIFTS) (P02358) | Escherichia coli | |
| 7 | G (AG) | 30S ribosomal protein S7 | polymer | 156 | 17637.4 | 1 | UniProt (P02359) Pfam (PF00177) | Escherichia coli | Small ribosomal subunit protein uS7 |
| 8 | H (AH) | 30S ribosomal protein S8 | polymer | 130 | 14146.6 | 1 | UniProt (C3SR12) Pfam (PF00410) UniProt (by SIFTS) (P0A7W7) | Escherichia coli | |
| 9 | I (AI) | 30S ribosomal protein S9 | polymer | 130 | 14886.3 | 1 | UniProt (C3SRY2) Pfam (PF00380) UniProt (by SIFTS) (P0A7X3) | Escherichia coli | |
| 10 | J (AJ) | 30S ribosomal protein S10 | polymer | 103 | 11755.6 | 1 | UniProt (C3SQT7) Pfam (PF00338) UniProt (by SIFTS) (P0A7R5) | Escherichia coli | |
| 11 | K (AK) | 30S ribosomal protein S11 | polymer | 129 | 13871.0 | 1 | UniProt (C3SR57) Pfam (PF00411) UniProt (by SIFTS) (P0A7R9) | Escherichia coli | |
| 12 | L (AL) | 30S ribosomal protein S12 | polymer | 124 | 13814.2 | 1 | UniProt (C3SQR7) Pfam (PF00164) UniProt (by SIFTS) (P0A7S3) | Escherichia coli | |
| 13 | M (AM) | 30S ribosomal protein S13 | polymer | 118 | 13128.5 | 1 | UniProt (C3SR52) Pfam (PF00416) UniProt (by SIFTS) (P0A7S9) | Escherichia coli | |
| 14 | N (AN) | 30S ribosomal protein S14 | polymer | 101 | 11606.6 | 1 | UniProt (C3SR07) Pfam (PF00253) UniProt (by SIFTS) (P0AG59) | Escherichia coli | |
| 15 | O (AO) | 30S ribosomal protein S15 | polymer | 89 | 10290.8 | 1 | UniProt (C3SSQ7) Pfam (PF00312) UniProt (by SIFTS) (P0ADZ4) | Escherichia coli | |
| 16 | P (AP) | 30S ribosomal protein S16 | polymer | 82 | 9207.6 | 1 | UniProt (C3SYP2) Pfam (PF00886) UniProt (by SIFTS) (P0A7T3) | Escherichia coli | |
| 17 | Q (AQ) | 30S ribosomal protein S17 | polymer | 84 | 9724.5 | 1 | UniProt (C3SQY7) Pfam (PF00366) UniProt (by SIFTS) (P0AG63) | Escherichia coli | |
| 18 | R (AR) | 30S ribosomal protein S18 | polymer | 75 | 9005.5 | 1 | UniProt (C3SFP7) Pfam (PF01084) UniProt (by SIFTS) (P0A7T7) | Escherichia coli | |
| 19 | S (AS) | 30S ribosomal protein S19 | polymer | 92 | 10455.4 | 1 | UniProt (C3SQW2) Pfam (PF00203) UniProt (by SIFTS) (P0A7U3) | Escherichia coli | |
| 20 | T (AT) | 30S ribosomal protein S20 | polymer | 87 | 9708.5 | 1 | UniProt (C3TRH7) Pfam (PF01649) UniProt (by SIFTS) (P0A7U7) | Escherichia coli | |
| 21 | U (AU) | 30S ribosomal protein S21 | polymer | 71 | 8524.0 | 1 | UniProt (C3STZ7) Pfam (PF01165) UniProt (by SIFTS) (P68679) | Escherichia coli | |
| 22 | V (AV) | mRNA | polymer | 53 | 17060.3 | 1 | synthetic construct | ||
| 23 | W (AW) | tRNA(fmet) P-site | polymer | 77 | 24832.9 | 1 | Escherichia coli | ||
| 24 | X (AX) | Phe-NH-tRNA(Phe) A-site | polymer | 76 | 24751.0 | 1 | GenBank (CP054236.1) | Escherichia coli | |
| 25 | Y (AY) | 30S ribosomal protein S1 | polymer | 557 | 61238.9 | 1 | UniProt (P0AG67) Pfam (PF00575) | Escherichia coli | Bacteriophage Q beta RNA-directed RNA polymerase subunit I,Small ribosomal subunit protein bS1 |
| 26 | Z (BA) | 23S ribosomal RNA | polymer | 2904 | 941820.6 | 1 | Escherichia coli | ||
| 27 | AA (BB) | 5S ribosomal RNA | polymer | 120 | 38790.1 | 1 | GenBank (CP035706.1) | Escherichia coli | |
| 28 | BA (BC) | 50S ribosomal protein L2 | polymer | 273 | 29923.6 | 1 | UniProt (P60422) Pfam (PF00181) Pfam (PF03947) | Escherichia coli | ribosomal protein uL2 |
| 29 | CA (BD) | 50S ribosomal protein L3 | polymer | 209 | 22291.6 | 1 | UniProt (P60438) Pfam (PF00297) | Escherichia coli | Large ribosomal subunit protein uL3 |
| 30 | DA (BE) | 50S ribosomal protein L4 | polymer | 201 | 22121.6 | 1 | UniProt (P60723) Pfam (PF00573) | Escherichia coli | ribosomal protein uL4 |
| 31 | EA (BF) | 50S ribosomal protein L5 | polymer | 179 | 20333.6 | 1 | UniProt (P62399) Pfam (PF00281) Pfam (PF00673) | Escherichia coli | ribosomal protein uL5 |
| 32 | FA (BG) | 50S ribosomal protein L6 | polymer | 177 | 18932.8 | 1 | UniProt (P0AG55) Pfam (PF00347) | Escherichia coli | ribosomal protein uL6 |
| 33 | GA (BH) | 50S ribosomal protein L9 | polymer | 149 | 15789.0 | 1 | UniProt (P0A7R1) Pfam (PF01281) Pfam (PF03948) | Escherichia coli | ribosomal protein bL9 |
| 34 | HA (BI) | 50S ribosomal protein L10 | polymer | 165 | 17736.6 | 1 | UniProt (A0A073UC57) Pfam (PF00466) UniProt (by SIFTS) (P0A7J3) | Escherichia coli | |
| 35 | IA (BJ) | 50S ribosomal protein L11 | polymer | 142 | 14894.4 | 1 | UniProt (P0A7J7) Pfam (PF03946) Pfam (PF00298) | Escherichia coli | ribosomal protein uL11 |
| 36 | JA (BK) | 50S ribosomal protein L13 | polymer | 142 | 16050.6 | 1 | UniProt (P0AA10) Pfam (PF00572) | Escherichia coli | ribosomal protein uL13 |
| 37 | KA (BL) | 50S ribosomal protein L14 | polymer | 123 | 13565.1 | 1 | UniProt (P0ADY3) Pfam (PF00238) | Escherichia coli | ribosomal protein uL14 |
| 38 | LA (BM) | 50S ribosomal protein L15 | polymer | 144 | 14994.4 | 1 | UniProt (P02413) Pfam (PF00828) | Escherichia coli | Large ribosomal subunit protein uL15 |
| 39 | MA (BN) | 50S ribosomal protein L16 | polymer | 136 | 15327.3 | 1 | UniProt (P0ADY7) Pfam (PF00252) | Escherichia coli | Large ribosomal subunit protein uL16 |
| 40 | NA (BO) | 50S ribosomal protein L17 | polymer | 127 | 14393.7 | 1 | UniProt (P0AG44) Pfam (PF01196) | Escherichia coli | ribosomal protein bL17 |
| 41 | OA (BP) | 50S ribosomal protein L18 | polymer | 117 | 12794.7 | 1 | UniProt (P0C018) Pfam (PF00861) | Escherichia coli | ribosomal protein uL18 |
| 42 | PA (BQ) | 50S ribosomal protein L19 | polymer | 115 | 13159.3 | 1 | UniProt (P0A7K6) Pfam (PF01245) | Escherichia coli | ribosomal protein bL19 |
| 43 | QA (BR) | 50S ribosomal protein L20 | polymer | 118 | 13528.0 | 1 | UniProt (P0A7L3) Pfam (PF00453) | Escherichia coli | ribosomal protein bL20 |
| 44 | RA (BS) | 50S ribosomal protein L21 | polymer | 103 | 11586.4 | 1 | UniProt (P0AG48) Pfam (PF00829) | Escherichia coli | ribosomal protein bL21 |
| 45 | SA (BT) | 50S ribosomal protein L22 | polymer | 110 | 12253.4 | 1 | UniProt (P61175) Pfam (PF00237) | Escherichia coli | ribosomal protein uL22 |
| 46 | TA (BU) | 50S ribosomal protein L23 | polymer | 100 | 11222.2 | 1 | UniProt (P0ADZ0) Pfam (PF00276) | Escherichia coli | ribosomal protein uL23 |
| 47 | UA (BV) | 50S ribosomal protein L24 | polymer | 104 | 11339.3 | 1 | UniProt (P60624) Pfam (PF00467) Pfam (PF17136) | Escherichia coli | ribosomal protein uL24 |
| 48 | VA (BW) | 50S ribosomal protein L25 | polymer | 94 | 10713.5 | 1 | UniProt (P68919) Pfam (PF01386) | Escherichia coli | ribosomal protein bL25 |
| 49 | WA (BX) | 50S ribosomal protein L27 | polymer | 85 | 9146.5 | 1 | UniProt (P0A7L8) Pfam (PF01016) | Escherichia coli | ribosomal protein bL27 |
| 50 | XA (BY) | 50S ribosomal protein L28 | polymer | 78 | 9027.6 | 1 | UniProt (P0A7M2) Pfam (PF00830) | Escherichia coli | ribosomal protein bL28 |
| 51 | YA (BZ) | 50S ribosomal protein L29 | polymer | 63 | 7286.5 | 1 | UniProt (P0A7M6) Pfam (PF00831) | Escherichia coli | ribosomal protein uL29 |
| 52 | ZA (B1) | 50S ribosomal protein L30 | polymer | 59 | 6554.8 | 1 | UniProt (P0AG51) Pfam (PF00327) | Escherichia coli | ribosomal protein uL30 |
| 53 | AB (B2) | 50S ribosomal protein L32 | polymer | 57 | 6463.4 | 1 | UniProt (P0A7N4) Pfam (PF01783) | Escherichia coli | ribosomal protein bL32 |
| 54 | BB (B3) | 50S ribosomal protein L33 | polymer | 55 | 6388.6 | 1 | UniProt (P0A7N9) Pfam (PF00471) | Escherichia coli | ribosomal protein bL33 |
| 55 | CB (B4) | 50S ribosomal protein L34 | polymer | 46 | 5397.5 | 1 | UniProt (P0A7P5) Pfam (PF00468) | Escherichia coli | ribosomal protein bL34 |
| 56 | DB (B5) | 50S ribosomal protein L35 | polymer | 65 | 7313.0 | 1 | UniProt (P0A7Q1) Pfam (PF01632) | Escherichia coli | Ribosomal protein A, ribosomal protein bL35 |
| 57 | EB (B6) | 50S ribosomal protein L36 | polymer | 50 | 5720.1 | 1 | UniProt (A0A2X1PTA3) Pfam (PF00444) UniProt (by SIFTS) (P0A7Q6) | Escherichia coli | |
| 58 | FB (B7) | 50S ribosomal protein L31 | polymer | 70 | 7887.1 | 1 | UniProt (U9XX47) Pfam (PF01197) UniProt (by SIFTS) (P0A7M9) | Escherichia coli | |
| 59 | GB (CN) | Non-template DNA strand | polymer | 39 | 12063.8 | 1 | synthetic construct | ||
| 60 | HB (CT) | Template DNA strand | polymer | 39 | 11872.6 | 1 | synthetic construct | ||
| 61 | IB, JB (CA, CB) | DNA-directed RNA polymerase subunit alpha | polymer | 329 | 36558.7 | 2 | UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) | Escherichia coli | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 62 | KB (CC) | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 1 | UniProt (P0A8V4) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) UniProt (by SIFTS) (P0A8V2) | Escherichia coli | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 63 | LB (CD) | DNA-directed RNA polymerase subunit beta' | polymer | 1407 | 155366.8 | 1 | UniProt (P0A8T8) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) UniProt (by SIFTS) (P0A8T7) | Escherichia coli | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 64 | MB (CE) | DNA-directed RNA polymerase subunit omega | polymer | 91 | 10249.5 | 1 | UniProt (P0A800) Pfam (PF01192) | Escherichia coli | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 65 | NB (CF) | Transcription termination/antitermination protein NusG | polymer | 181 | 20502.4 | 1 | UniProt (P0AFG1) Pfam (PF02357) Pfam (PF00467) UniProt (by SIFTS) (P0AFG0) | Escherichia coli | |
| 66 | AC, AD, AE, AF, AG... (AA, BA, CD, AI, AW...) | MAGNESIUM ION | non-polymer | 24.3 | 479 | Chemie (MG) | |||
| 67 | IH (AX) | PHENYLALANINE | non-polymer | 165.2 | 1 | Chemie (PHE) | |||
| 68 | AU, CU, DU, ZT (B7, CD, B6) | ZINC ION | non-polymer | 65.4 | 4 | Chemie (ZN) |
Sequence modifications
AE: 1 - 167 (UniProt: A0A090BZW5)
BM: 1 - 144 (UniProt: P02413)
CF: 1 - 181 (UniProt: P0AFG1)
| PDB | External Database | Details |
|---|---|---|
| Cys 9 | Gly 9 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Val 77 | Ile 77 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Ala 121 | Lys 121 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 66 |
| Total formula weight | 2733415.3 | |
| Non-Polymers* | Number of molecules | 484 |
| Total formula weight | 12068.9 | |
| All* | Total formula weight | 2745484.3 |
