6ZSN
Crystal structure of rsGCaMP double mutant Ile80His/Val116Ile in the OFF state (illuminated)
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Green fluorescent protein,Green fluorescent protein,Calmodulin | polymer | 420 | 47325.0 | 1 | UniProt (P42212) UniProt (K4DIE3) Pfam (PF01353) Pfam (PF13499) In PDB | Aequorea victoria (Jellyfish) | |
2 | A | FORMIC ACID | non-polymer | 46.0 | 6 | Chemie (FMT) | |||
3 | A | CALCIUM ION | non-polymer | 40.1 | 4 | Chemie (CA) | |||
4 | A | SODIUM ION | non-polymer | 23.0 | 1 | Chemie (NA) | |||
5 | water | water | 18.0 | 108 | Chemie (HOH) |
Sequence modifications
A: 62 - 151 (UniProt: P42212)
A: 160 - 302 (UniProt: P42212)
A: 303 - 451 (UniProt: K4DIE3)
PDB | External Database | Details |
---|---|---|
Met 36 | - | initiating methionine |
Val 37 | - | expression tag |
Asp 38 | - | expression tag |
Ser 39 | - | expression tag |
Ser 40 | - | expression tag |
Arg 41 | - | expression tag |
Arg 42 | - | expression tag |
Lys 43 | - | expression tag |
Trp 44 | - | expression tag |
Asn 45 | - | expression tag |
Lys 46 | - | expression tag |
Thr 47 | - | expression tag |
Gly 48 | - | expression tag |
His 49 | - | expression tag |
Ala 50 | - | expression tag |
Val 51 | - | expression tag |
Arg 52 | - | expression tag |
Ala 53 | - | expression tag |
Ile 54 | - | expression tag |
Gly 55 | - | expression tag |
Arg 56 | - | expression tag |
Leu 57 | - | expression tag |
Ser 58 | - | expression tag |
Ser 59 | - | expression tag |
Leu 60 | - | expression tag |
Glu 61 | - | expression tag |
Lys 66 | Met 153 | conflict |
Ala 76 | Val 163 | conflict |
His 80 | Ile 167 | conflict |
Gly 88 | Ser 175 | conflict |
Tyr 93 | Asp 180 | conflict |
Ile 116 | Thr 203 | conflict |
Lys 119 | Ala 206 | conflict |
Leu 144 | His 231 | conflict |
PDB | External Database | Details |
---|---|---|
Gly 152 | - | linker |
Gly 153 | - | linker |
Thr 154 | - | linker |
Gly 155 | - | linker |
Gly 156 | - | linker |
Ser 157 | - | linker |
Met 158 | - | linker |
Val 159 | - | linker |
- | Phe 64 | deletion |
- | Ser 65 | deletion |
Leu 222 | Tyr 66 | conflict |
Pia 224 | Gly 67 | conflict |
Leu 227 | Gln 69 | conflict |
Ile 251 | Val 93 | conflict |
PDB | External Database | Details |
---|---|---|
Thr 373 | Ile 354 | conflict |
Tyr 381 | Asp 362 | conflict |
Leu 452 | - | expression tag |
Glu 453 | - | expression tag |
His 454 | - | expression tag |
His 455 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 47325.0 | |
Non-Polymers* | Number of molecules | 11 |
Total formula weight | 459.5 | |
All* | Total formula weight | 47784.4 |