6ZOA

Partially induced AcrB T protomer and DDM binding to the TM8/PC2 pathway of AcrB L2 protomer

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C	Multidrug efflux pump subunit AcrB	polymer	1057	114736.3	3	UniProt (P31224) Pfam (PF00873) In PDB	Escherichia coli K-12	AcrAB-TolC multidrug efflux pump subunit AcrB,Acridine resistance protein B
2	D, E	DARPIN	polymer	169	18317.6	2		synthetic construct
3	C, A, B	DODECYL-BETA-D-MALTOSIDE	non-polymer		510.6	6	Chemie (LMT)
4	A, B	TETRADECANE	non-polymer		198.4	4	Chemie (C14)
5	C, A, B	DECYLAMINE-N,N-DIMETHYL-N-OXIDE	non-polymer		201.3	7	Chemie (DDQ)
6	C, A, B	HEXANE	non-polymer		86.2	4	Chemie (HEX)
7	A	DECANE	non-polymer		142.3	1	Chemie (D10)
8	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
9	A	PENTANE	non-polymer		72.1	1	Chemie (LNK)
10	B, A	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
11	B, C	N-OCTANE	non-polymer		114.2	2	Chemie (OCT)
12	B	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
13	C	PHOSPHATIDYLETHANOLAMINE	non-polymer		734.0	2	Chemie (PTY)
14	C	DODECANE	non-polymer		170.3	1	Chemie (D12)
15		water	water		18.0	275	Chemie (HOH)

Sequence modifications

A, B, C: 1 - 1049 (UniProt: P31224)

PDB	External Database	Details
Leu 1050	-	expression tag
Glu 1051	-	expression tag
His 1052	-	expression tag
His 1053	-	expression tag
His 1054	-	expression tag
His 1055	-	expression tag
His 1056	-	expression tag
His 1057	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	380844.0
Non-Polymers*	Number of molecules	33
	Total formula weight	8066.5
All*	Total formula weight	388910.5

*Water molecules are not included.