6ZLJ
Crystal Structure of UDP-Glucuronic acid 4-epimerase Y149F mutant from Bacillus cereus in complex with UDP-4-DEOXY-4-FLUORO-Glucuronic acid and NAD
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Epimerase domain-containing protein | polymer | 327 | 36511.0 | 2 | UniProt (J8BY31) In PDB | Bacillus cereus | |
2 | A, B | NICOTINAMIDE-ADENINE-DINUCLEOTIDE | non-polymer | 663.4 | 2 | Chemie (NAD) | |||
3 | A, B | (2~{R},3~{S},4~{R},5~{R},6~{R})-6-[[[(2~{R},3~{S},4~{R},5~{R})-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidany l)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3-fluoranyl-4,5-bis(oxidanyl)oxane-2-carboxylic acid | non-polymer | 582.3 | 2 | Chemie (UGF) | |||
4 | water | water | 18.0 | 168 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 317 (UniProt: J8BY31)
PDB | External Database | Details |
---|---|---|
Phe 149 | Tyr 149 | engineered mutation |
Ala 318 | - | expression tag |
Ala 319 | - | expression tag |
Trp 320 | - | expression tag |
Ser 321 | - | expression tag |
His 322 | - | expression tag |
Pro 323 | - | expression tag |
Gln 324 | - | expression tag |
Phe 325 | - | expression tag |
Glu 326 | - | expression tag |
Lys 327 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 73021.9 | |
Non-Polymers* | Number of molecules | 4 |
Total formula weight | 2491.4 | |
All* | Total formula weight | 75513.3 |