6ZHY

Cryo-EM structure of the regulatory linker of ALC1 bound to the nucleosome's acidic patch: hexasome class.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	Histone H3	polymer	136	15403.1	2	UniProt (A0A310TTQ1) Pfam (PF00125) UniProt (by SIFTS) (P84233)	Xenopus laevis (African clawed frog)
2	B, F (B, F)	Histone H4	polymer	103	11394.4	2	UniProt (P62799) Pfam (PF15511)	Xenopus laevis (African clawed frog)
3	C (C)	Histone H2A type 1	polymer	130	14109.4	1	UniProt (P06897) Pfam (PF00125) Pfam (PF16211)	Xenopus laevis (African clawed frog)
4	D (D)	Histone H2B 1.1	polymer	123	13655.9	1	UniProt (P02281) Pfam (PF00125)	Xenopus laevis (African clawed frog)	H2B1.1
5	G (I)	DNA (110-MER) Widom 601 sequence	polymer	145	44520.4	1		synthetic construct
6	H (J)	DNA (110-MER) Widom 601 sequence	polymer	145	44991.7	1		synthetic construct
7	I (K)	Chromodomain-helicase-DNA-binding protein 1-like	polymer	37	4073.7	1	UniProt (Q86WJ1)	Homo sapiens	Amplified in liver cancer protein 1

Sequence modifications

A, E: 0 - 135 (UniProt: A0A310TTQ1)

PDB	External Database	Details
Ala 110	Cys 111	engineered mutation

C: 0 - 129 (UniProt: P06897)

PDB	External Database	Details
Arg 99	Gly 100	conflict
Ser 123	Ala 124	conflict

D: 1 - 122 (UniProt: P02281)

PDB	External Database	Details
Met 0	-	initiating methionine
Thr 29	Ser 33	conflict

K: 604 - 639 (UniProt: Q86WJ1)

PDB	External Database	Details
Lys 640	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	174946.1
All*	Total formula weight	174946.1

*Water molecules are not included.