6ZD0

Disulfide-locked early prepore intermedilysin-CD59

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E	Thiol-activated cytolysin	polymer	535	59101.0	3	UniProt (Q9LCB8) Pfam (PF01289) Pfam (PF17440) In PDB	Streptococcus intermedius
2	B, D, F	CD59 glycoprotein	polymer	79	9204.4	3	UniProt (P13987) Pfam (PF00021) In PDB	Homo sapiens (Human)	1F5 antigen,20 kDa homologous restriction factor,HRF20,MAC-inhibitory protein,MAC-IP,MEM43 antigen,Membrane attack complex inhibition factor,MACIF,Membrane inhibitor of reactive lysis,MIRL,Protectin

Sequence modifications

A, C, E: 34 - 532 (UniProt: Q9LCB8)

PDB	External Database	Details
Met -2	-	initiating methionine
Gly -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag
His 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
Gly 8	-	expression tag
Met 9	-	expression tag
Ala 10	-	expression tag
Ser 11	-	expression tag
Met 12	-	expression tag
Thr 13	-	expression tag
Gly 14	-	expression tag
Gly 15	-	expression tag
Gln 16	-	expression tag
Gln 17	-	expression tag
Met 18	-	expression tag
Gly 19	-	expression tag
Arg 20	-	expression tag
Asp 21	-	expression tag
Leu 22	-	expression tag
Tyr 23	-	expression tag
Asp 24	-	expression tag
Asp 25	-	expression tag
Asp 26	-	expression tag
Asp 27	-	expression tag
Lys 28	-	expression tag
Asp 29	-	expression tag
Arg 30	-	expression tag
Trp 31	-	expression tag
Gly 32	-	expression tag
Ser 33	-	expression tag
Cys 104	Ile 104	engineered mutation
Cys 244	Gly 244	engineered mutation

B, D, F: 1 - 77 (UniProt: P13987)

PDB	External Database	Details
Met 0	-	initiating methionine
Cys 78	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	204916.2
All*	Total formula weight	204916.2

*Water molecules are not included.