6Z9Q

Transcription termination intermediate complex 2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (G)	Transcription termination/antitermination protein NusG	polymer	181	20560.5	1	UniProt (C3SID2) Pfam (PF02357) Pfam (PF00467) UniProt (by SIFTS) (P0AFG0)	Escherichia coli
2	B, C, D, E, F... (f, a, b, c, d...)	Transcription termination factor Rho	polymer	419	47070.2	6	UniProt (A0A0A0GPI6) Pfam (PF07498) Pfam (PF07497) Pfam (PF00006) UniProt (by SIFTS) (P0AG30)	Escherichia coli	ATP-dependent helicase Rho
3	H (A)	Transcription termination/antitermination protein NusA	polymer	497	55060.8	1	UniProt (C3SSN7) Pfam (PF08529) Pfam (PF00575) Pfam (PF13184) Pfam (PF14520) UniProt (by SIFTS) (P0AFF6)	Escherichia coli
4	I, J (U, V)	DNA-directed RNA polymerase subunit alpha	polymer	329	36558.7	2	UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118)	Escherichia coli	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
5	K (W)	DNA-directed RNA polymerase subunit omega	polymer	91	10249.5	1	UniProt (P0A800) Pfam (PF01192)	Escherichia coli	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
6	L (X)	DNA-directed RNA polymerase subunit beta	polymer	1342	150820.9	1	UniProt (P0A8V4) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) UniProt (by SIFTS) (P0A8V2)	Escherichia coli	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
7	M (Y)	DNA-directed RNA polymerase subunit beta'	polymer	1416	156537.0	1	UniProt (C3SIA2) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) UniProt (by SIFTS) (P0A8T7)	Escherichia coli	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
8	N (K)	non template strand	polymer	50	15542.0	1		synthetic construct
9	O (L)	template DNA	polymer	50	15211.7	1		synthetic construct
10	P (R)	rut RNA	polymer	99	31693.9	1	GenBank (1227111474)	synthetic construct
11	CA, Q, T, W, Z (e, f, b, c, d)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	5	Chemie (ADP)
12	AA, DA, FA, R, U... (d, e, Y, f, b...)	MAGNESIUM ION	non-polymer		24.3	6	Chemie (MG)
13	BA, EA, S, V, Y (d, e, f, b, c)	BERYLLIUM TRIFLUORIDE ION	non-polymer		66.0	5	Chemie (BEF)
14	GA, HA (Y)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
15	IA, JA, KA, LA, MA (f, b, c, d, e)	water	water		18.0	15	Chemie (HOH)

Sequence modifications

A: 1 - 495 (UniProt: C3SSN7)

PDB	External Database	Details
Gly -1	-	expression tag
Ala 0	-	expression tag

Y: 1 - 1407 (UniProt: C3SIA2)

PDB	External Database	Details
Leu 1408	-	expression tag
Glu 1409	-	expression tag
Val 1410	-	expression tag
His 1411	-	expression tag
His 1412	-	expression tag
His 1413	-	expression tag
His 1414	-	expression tag
His 1415	-	expression tag
His 1416	-	expression tag

R: 4 - 76 (GenBank: 1227111474)

PDB	External Database	Details
G 1	-	expression tag
G 2	-	expression tag
G 3	-	expression tag
U 72	-	insertion
U 77	-	expression tag
U 78	-	expression tag
A 79	-	expression tag
A 80	-	expression tag
A 81	-	expression tag
C 82	-	expression tag
C 83	-	expression tag
A 84	-	expression tag
C 85	-	expression tag
A 86	-	expression tag
C 87	-	expression tag
C 88	-	expression tag
U 89	-	expression tag
G 90	-	expression tag
G 91	-	expression tag
C 92	-	expression tag
G 93	-	expression tag
U 94	-	expression tag
G 95	-	expression tag
U 96	-	expression tag
G 97	-	expression tag
G 98	-	expression tag
C 99	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	16
	Total formula weight	811214.7
Non-Polymers*	Number of molecules	18
	Total formula weight	2742.7
All*	Total formula weight	813957.4

*Water molecules are not included.