6Z3O

The E74Q mutant of Small Alarmone Hydrolase SAH from Pseudomonas aeruginosa PAO1

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (AAA, BBB, CCC, DDD)	Small Alarmone Hydrolase	polymer	205	23107.8	4	UniProt (Q9I686) Pfam (PF13328)	Pseudomonas aeruginosa PAO1
2	E, F, G, H (AAA, BBB, CCC, DDD)	MANGANESE (II) ION	non-polymer		54.9	4	Chemie (MN)
3	I, J, K, L (AAA, BBB, CCC, DDD)	water	water		18.0	526	Chemie (HOH)

Sequence modifications

AAA, BBB, CCC, DDD: 1 - 184 (UniProt: Q9I686)

PDB	External Database	Details
Met -20	-	initiating methionine
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Gln 74	Glu 74	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	92431.4
Non-Polymers*	Number of molecules	4
	Total formula weight	219.8
All*	Total formula weight	92651.1

*Water molecules are not included.