6Z2A

Structure of Clr4 mutant - F256A/F310A/F427A bound to SAH

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Histone-lysine N-methyltransferase, H3 lysine-9 specific	polymer	296	33366.2	1	UniProt (O60016) Pfam (PF05033) Pfam (PF00856) In PDB	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	Cryptic loci regulator 4,Histone H3-K9 methyltransferase,HKMT,Lysine N-methyltransferase 1,Protein lysine methyltransferase clr4,PKMT
2	B	Histone-lysine N-methyltransferase, H3 lysine-9 specific	polymer	294	33194.1	1	UniProt (O60016) Pfam (PF05033) Pfam (PF00856) In PDB	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	Cryptic loci regulator 4,Histone H3-K9 methyltransferase,HKMT,Lysine N-methyltransferase 1,Protein lysine methyltransferase clr4,PKMT
3	A, B	ZINC ION	non-polymer		65.4	8	Chemie (ZN)
4	A, B	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	2	Chemie (SAH)
5	A, B	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6		water	water		18.0	22	Chemie (HOH)

Sequence modifications

A: 195 - 490 (UniProt: O60016)

PDB	External Database	Details
Ala 256	Phe 256	engineered mutation
Ala 310	Phe 310	engineered mutation
Ala 427	Phe 427	engineered mutation

B: 196 - 489 (UniProt: O60016)

PDB	External Database	Details
Ala 256	Phe 256	engineered mutation
Ala 310	Phe 310	engineered mutation
Ala 427	Phe 427	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	66560.3
Non-Polymers*	Number of molecules	12
	Total formula weight	1340.7
All*	Total formula weight	67901.0

*Water molecules are not included.