6Z22

Crystal structure of deacylation mutant KPC-4 (E166Q)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Beta-lactamase	polymer	290	30823.6	1	UniProt (B1PL86) Pfam (PF00144) Pfam (PF13354) UniProt (by SIFTS) (Q9F663)	Klebsiella pneumoniae
2	B, C, D, F (A)	SULFATE ION	non-polymer		96.1	4	Chemie (SO4)
3	E (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
4	G (A)	water	water		18.0	306	Chemie (HOH)

Sequence modifications

A: 25 - 295 (UniProt: B1PL86)

PDB	External Database	Details
Met 4	-	initiating methionine
Gly 5	-	expression tag
Ser 6	-	expression tag
Ser 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
His 11	-	expression tag
His 12	-	expression tag
His 13	-	expression tag
Ser 14	-	expression tag
Ser 15	-	expression tag
Gly 16	-	expression tag
Leu 17	-	expression tag
Val 18	-	expression tag
Pro 19	-	expression tag
Arg 20	-	expression tag
Gly 21	-	expression tag
Ser 22	-	expression tag
His 23	-	expression tag
Met 24	-	expression tag
Gln 166	Glu 165	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	30823.6
Non-Polymers*	Number of molecules	5
	Total formula weight	476.3
All*	Total formula weight	31300.0

*Water molecules are not included.