6Z1S

Structure of Polyphenol Oxidase (mutant G292N) from Thermothelomyces thermophila

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Tyrosinase-like protein	polymer	425	46573.6	1	UniProt (G2QLD3) Pfam (PF00264)	Thermothelomyces thermophilus ATCC 42464
2	B (B)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
3	C, E (A)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)
4	D (A)	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	1	Chemie (MPD)
5	F, H, I, J, K... (A)	GLYCINE	non-polymer		75.1	6	Chemie (GLY)
6	G (A)	SERINE	non-polymer		105.1	1	Chemie (SER)
7	M, N (A)	COPPER (II) ION	non-polymer		63.5	2	Chemie (CU)
8	O (A)	water	water		18.0	359	Chemie (HOH)

Sequence modifications

A: 3 - 404 (UniProt: G2QLD3)

PDB	External Database	Details
Glu 1	-	expression tag
Phe 2	-	expression tag
Asn 292	Gly 312	engineered mutation
Glu 405	-	expression tag
Gln 406	-	expression tag
Lys 407	-	expression tag
Leu 408	-	expression tag
Ile 409	-	expression tag
Ser 410	-	expression tag
Glu 411	-	expression tag
Glu 412	-	expression tag
Asp 413	-	expression tag
Leu 414	-	expression tag
Asn 415	-	expression tag
Ser 416	-	expression tag
Ala 417	-	expression tag
Val 418	-	expression tag
Asp 419	-	expression tag
His 420	-	expression tag
His 421	-	expression tag
His 422	-	expression tag
His 423	-	expression tag
His 424	-	expression tag
His 425	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	46573.6
Branched	Number of molecules	1
	Total formula weight	424.4
Non-Polymers*	Number of molecules	12
	Total formula weight	1243.2
All*	Total formula weight	48241.1

*Water molecules are not included.