6YWI

Structure of Chloroflexus aggregans flavin based fluorescent protein (CagFbFP) Q148E variant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Multi-sensor hybrid histidine kinase	polymer	113	12401.9	2	UniProt (B8GAY9) Pfam (PF13426)	Chloroflexus aggregans (strain MD-66 / DSM 9485)
2	C, F (A, B)	FLAVIN MONONUCLEOTIDE	non-polymer		456.3	2	Chemie (FMN)
3	D (A)	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	1	Chemie (MES)
4	E, G (A, B)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
5	H, I (A, B)	water	water		18.0	191	Chemie (HOH)

Sequence modifications

A, B: 47 - 153 (UniProt: B8GAY9)

PDB	External Database	Details
Ala 85	Cys 85	engineered mutation
Glu 148	Gln 148	engineered mutation
His 154	-	expression tag
His 155	-	expression tag
His 156	-	expression tag
His 157	-	expression tag
His 158	-	expression tag
His 159	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	24803.7
Non-Polymers*	Number of molecules	5
	Total formula weight	1292.1
All*	Total formula weight	26095.8

*Water molecules are not included.