6YT4

Crystal structure of the N112A mutant of the light-driven sodium pump KR2 in the pentameric form, pH 8.0

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, D, E, C	Sodium pumping rhodopsin	polymer	288	32568.6	5	UniProt (N0DKS8) Pfam (PF01036) In PDB	Dokdonia eikasta
2	A, C, D, B, E	(2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate	non-polymer		356.5	5	Chemie (OLC)
3	E, B, A, C, D	OLEIC ACID	non-polymer		282.5	16	Chemie (OLA)
4	E, B, D, A, C	EICOSANE	non-polymer		282.5	28	Chemie (LFA)
5	B, E, D, A, C	octyl beta-D-glucopyranoside	non-polymer		292.4	5	Chemie (BOG)
6	B, E, A, C, D	RETINAL	non-polymer		284.4	5	Chemie (RET)
7	B, E, A, C, D	SODIUM ION	non-polymer		23.0	5	Chemie (NA)
8		water	water		18.0	286	Chemie (HOH)

Sequence modifications

A, B, D, E, C: 1 - 280 (UniProt: N0DKS8)

PDB	External Database	Details
Ala 112	Asn 112	engineered mutation
Leu 281	-	expression tag
Glu 282	-	expression tag
His 283	-	expression tag
His 284	-	expression tag
His 285	-	expression tag
His 286	-	expression tag
His 287	-	expression tag
His 288	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	162842.8
Non-Polymers*	Number of molecules	64
	Total formula weight	17212.4
All*	Total formula weight	180055.2

*Water molecules are not included.