6YSA

Crystal structure of Arabidopsis thaliana legumain isoform beta in zymogen state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	Vacuolar-processing enzyme beta-isozyme	polymer	455	50576.8	12	UniProt (Q39044) Pfam (PF01650) Pfam (PF20985) In PDB	Arabidopsis thaliana (thale cress)	Asparaginyl endopeptidase beta-VPE,Beta-VPE
2	M, N, O, P, Q...	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	12	In PDB GlyTouCan (G42666HT)
3	A, F, J, B, K...	SULFATE ION	non-polymer		96.1	66	Chemie (SO4)
4	E	CITRIC ACID	non-polymer		192.1	1	Chemie (CIT)
5		water	water		18.0	2252	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F, G, H, I, J, K, L: 47 - 486 (UniProt: Q39044)

PDB	External Database	Details
Ser 32	-	expression tag
Leu 33	-	expression tag
Glu 34	-	expression tag
His 35	-	expression tag
His 36	-	expression tag
His 37	-	expression tag
His 38	-	expression tag
His 39	-	expression tag
His 40	-	expression tag
Glu 41	-	expression tag
Asn 42	-	expression tag
Leu 43	-	expression tag
Tyr 44	-	expression tag
Phe 45	-	expression tag
Gln 46	-	expression tag
Snn 168	Asp 168	modified residue
Ala 211	Cys 211	conflict

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	606921.5
Branched	Number of molecules	12
	Total formula weight	5092.8
Non-Polymers*	Number of molecules	67
	Total formula weight	6532.3
All*	Total formula weight	618546.6

*Water molecules are not included.