6YN2

Crystal structure of Renilla reniformis luciferase variant RLuc8-W121F/E144Q in complex with a coelenteramide (the postcatalytic enzyme-product complex)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Coelenterazine h 2-monooxygenase	polymer	317	36907.2	2	UniProt (P27652) Pfam (PF00561)	Renilla reniformis (Sea pansy)	Renilla-luciferin 2-monooxygenase,Renilla-type luciferase
2	C, F (A, B)	N-[3-BENZYL-5-(4-HYDROXYPHENYL)PYRAZIN-2-YL]-2-(4-HYDROXYPHENYL)ACETAMIDE	non-polymer		411.5	2	Chemie (CEI)
3	D (A)	ACETATE ION	non-polymer		59.0	1	Chemie (ACT)
4	E (B)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
5	G (B)	POTASSIUM ION	non-polymer		39.1	1	Chemie (K)
6	H, I (A, B)	water	water		18.0	507	Chemie (HOH)

Sequence modifications

A, B: 1 - 311 (UniProt: P27652)

PDB	External Database	Details
Thr 55	Ala 55	conflict
Phe 121	Trp 121	engineered mutation
Ala 124	Cys 124	conflict
Ala 130	Ser 130	conflict
Arg 136	Lys 136	conflict
Met 143	Ala 143	conflict
Gln 144	Glu 144	engineered mutation
Val 185	Met 185	conflict
Leu 253	Met 253	conflict
Leu 287	Ser 287	conflict
His 312	-	expression tag
His 313	-	expression tag
His 314	-	expression tag
His 315	-	expression tag
His 316	-	expression tag
His 317	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	73814.3
Non-Polymers*	Number of molecules	5
	Total formula weight	1013.1
All*	Total formula weight	74827.5

*Water molecules are not included.