6YMV

Cryo-EM structure of yeast mitochondrial RNA polymerase partially-melted transcription initiation complex (PmIC)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	Mitochondrial transcription factor 1	polymer	354	41151.2	1	UniProt (P14908) Pfam (PF00398)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	Mitochondrial transcription factor mtTFB,Mitochondrial-specificity factor,RF1023
2	B (A)	DNA-directed RNA polymerase, mitochondrial	polymer	1262	143282.7	1	UniProt (P13433) Pfam (PF14700) Pfam (PF00940)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
3	C (N)	DNA (33-MER) NON-TEMPLATE	polymer	33	10248.7	1		Saccharomyces cerevisiae
4	D (T)	DNA (33-MER) template	polymer	33	10047.5	1		Saccharomyces cerevisiae

Sequence modifications

B: 2 - 341 (UniProt: P14908)

PDB	External Database	Details
Met -12	-	initiating methionine
Gly -11	-	expression tag
Gly -10	-	expression tag
Ser -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Gly -2	-	expression tag
Met -1	-	expression tag
Ala 0	-	expression tag
Ser 1	-	expression tag

A: 100 - 1351 (UniProt: P13433)

PDB	External Database	Details
Gly 90	-	expression tag
Ala 91	-	expression tag
Met 92	-	expression tag
Gly 93	-	expression tag
Ser 94	-	expression tag
Gly 95	-	expression tag
Ile 96	-	expression tag
Gln 97	-	expression tag
Arg 98	-	expression tag
Pro 99	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	204730.0
All*	Total formula weight	204730.0

*Water molecules are not included.