6YBB
Crystal structure of a native BcsE (217-523) - BcsR-BcsQ (R156E mutant) complex with c-di-GMP and ATP bound
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Bacterial cellulose secretion regulator BcsQ, R156E mutant | polymer | 250 | 27932.8 | 2 | UniProt (A0A0B1KWQ0) Pfam (PF06564) UniProt (by SIFTS) (P0DP92) | Escherichia coli | Cellulose biosynthesis protein BcsQ,Cellulose synthase operon protein YhjQ,Cellulose synthase,putative, Bacterial cellulose synthesis subunit Q |
| 2 | C, D (C, D) | Bacterial cellulose secretion regulator BcsR | polymer | 67 | 7452.4 | 2 | UniProt (J7QAC9) Pfam (PF10945) UniProt (by SIFTS) (P0ADJ3) | Escherichia coli | FIG004405: Putative cytoplasmic protein,Protein YhjR,Protein of uncharacterized function (DUF2629), Bacterial cellulose synthesis subunit R |
| 3 | E, F (E, F) | Bacterial cellulose secretion regulator BcsE, residues 217-523 | polymer | 310 | 34958.3 | 2 | UniProt (P37657) Pfam (PF10995) | Escherichia coli (strain K12) | Cellulose biosynthesis protein BcsE, Bacterial cellulose synthesis subunit E |
| 4 | G, I (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 5 | H, J (A, B) | ADENOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 2 | Chemie (ATP) | |||
| 6 | K, L, N, O (E, F) | 9,9'-[(2R,3R,3aS,5S,7aR,9R,10R,10aS,12S,14aR)-3,5,10,12-tetrahydroxy-5,12-dioxidooctahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-2,9-diyl]bis(2-amino-1,9-dihydro-6H-purin-6-one) | non-polymer | 690.4 | 4 | Chemie (C2E) | |||
| 7 | M, P (E, F) | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
| 8 | Q, R, S, T, U (A, B, D, E, F) | water | water | 18.0 | 22 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 250 (UniProt: A0A0B1KWQ0)
C, D: 1 - 62 (UniProt: J7QAC9)
E, F: 217 - 523 (UniProt: P37657)
| PDB | External Database | Details |
|---|---|---|
| Glu 156 | Arg 156 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly -4 | - | expression tag |
| Pro -3 | - | expression tag |
| Met -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met 214 | - | initiating methionine |
| Gly 215 | - | expression tag |
| Ser 216 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 140686.9 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 4008.8 | |
| All* | Total formula weight | 144695.7 |
