6Y7E
Pseudomonas stutzeri nitrous oxide reductase mutant, H494A
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Nitrous-oxide reductase | polymer | 646 | 71891.5 | 2 | UniProt (P19573) Pfam (PF18793) Pfam (PF18764) Pfam (PF00116) | Pseudomonas stutzeri | N(2)OR,N2O reductase |
| 2 | C, G, L, O (A, B) | FORMIC ACID | non-polymer | 46.0 | 4 | Chemie (FMT) | |||
| 3 | D, E, M (A, B) | ZINC ION | non-polymer | 65.4 | 3 | Chemie (ZN) | |||
| 4 | F (A) | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 122.1 | 1 | Chemie (TRS) | |||
| 5 | H, N (A, B) | 2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 282.3 | 2 | Chemie (B3P) | |||
| 6 | I, P (A, B) | SODIUM ION | non-polymer | 23.0 | 2 | Chemie (NA) | |||
| 7 | J, R (A, B) | CHLORIDE ION | non-polymer | 35.5 | 2 | Chemie (CL) | |||
| 8 | K, S (A, B) | DINUCLEAR COPPER ION | non-polymer | 127.1 | 2 | Chemie (CUA) | |||
| 9 | Q (B) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
| 10 | T, U (A, B) | water | water | 18.0 | 1095 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 638 (UniProt: P19573)
| PDB | External Database | Details |
|---|---|---|
| Ala 494 | His 494 | engineered mutation |
| Trp 639 | - | expression tag |
| Ser 640 | - | expression tag |
| His 641 | - | expression tag |
| Pro 642 | - | expression tag |
| Gln 643 | - | expression tag |
| Phe 644 | - | expression tag |
| Glu 645 | - | expression tag |
| Lys 646 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 143783.0 | |
| Non-Polymers* | Number of molecules | 17 |
| Total formula weight | 1478.3 | |
| All* | Total formula weight | 145261.3 |
