6Y7B
X-ray structure of the Haloalkane dehalogenase HaloTag7 labeled with a chloroalkane-carbopyronine fluorophore substrate
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C, D, E | Haloalkane dehalogenase | polymer | 293 | 33226.0 | 5 | UniProt (P0A3G3) Pfam (PF00561) In PDB | Rhodococcus sp. | |
2 | A, B, C, D, E | 4-[2-[2-(6-chloranylhexoxy)ethoxy]ethylcarbamoyl]-2-[3-(dimethylamino)-6-(dimethyl-$l^{4}-azanylidene)-10,10-dimethyl-anthracen-9-yl]benzoic acid | non-polymer | 663.3 | 5 | Chemie (OEK) | |||
3 | A, B, C, D, E | CHLORIDE ION | non-polymer | 35.5 | 5 | Chemie (CL) |
Sequence modifications
A, B, C, D, E: 4 - 293 (UniProt: P0A3G3)
PDB | External Database | Details |
---|---|---|
Gly 3 | - | expression tag |
Val 47 | Leu 47 | engineered mutation |
Thr 58 | Ser 58 | engineered mutation |
Gly 78 | Asp 78 | engineered mutation |
Phe 87 | Tyr 87 | engineered mutation |
Met 88 | Leu 88 | engineered mutation |
Phe 128 | Cys 128 | engineered mutation |
Thr 155 | Ala 155 | engineered mutation |
Lys 160 | Glu 160 | engineered mutation |
Val 167 | Ala 167 | engineered mutation |
Thr 172 | Ala 172 | engineered mutation |
Met 175 | Lys 175 | engineered mutation |
Gly 176 | Cys 176 | engineered mutation |
Asn 195 | Lys 195 | engineered mutation |
Glu 224 | Ala 224 | engineered mutation |
Asp 227 | Asn 227 | engineered mutation |
Lys 257 | Glu 257 | engineered mutation |
Ala 264 | Thr 264 | engineered mutation |
Asn 272 | His 272 | engineered mutation |
Leu 273 | Tyr 273 | engineered mutation |
Ser 291 | Pro 291 | engineered mutation |
Thr 292 | Ala 292 | engineered mutation |
Glu 294 | - | expression tag |
Ile 295 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 5 |
Total formula weight | 166129.9 | |
Non-Polymers* | Number of molecules | 10 |
Total formula weight | 3493.6 | |
All* | Total formula weight | 169623.5 |