6Y72

Pseudomonas stutzeri nitrous oxide reductase mutant, H178A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Nitrous-oxide reductase	polymer	646	71891.5	2	UniProt (P19573) Pfam (PF18793) Pfam (PF18764) Pfam (PF00116) In PDB	Pseudomonas stutzeri	N(2)OR,N2O reductase
2	A, B	2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		282.3	2	Chemie (B3P)
3	A	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
4	A, B	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
5	A, B	FORMIC ACID	non-polymer		46.0	7	Chemie (FMT)
6	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
7	A, B	DINUCLEAR COPPER ION	non-polymer		127.1	2	Chemie (CUA)
8	B	POTASSIUM ION	non-polymer		39.1	1	Chemie (K)
9		water	water		18.0	1045	Chemie (HOH)

Sequence modifications

A, B: 1 - 638 (UniProt: P19573)

PDB	External Database	Details
Ala 178	His 178	engineered mutation
Trp 639	-	expression tag
Ser 640	-	expression tag
His 641	-	expression tag
Pro 642	-	expression tag
Gln 643	-	expression tag
Phe 644	-	expression tag
Glu 645	-	expression tag
Lys 646	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	143783.0
Non-Polymers*	Number of molecules	17
	Total formula weight	1341.7
All*	Total formula weight	145124.7

*Water molecules are not included.