6Y71

Pseudomonas stutzeri nitrous oxide reductase mutant, H130A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Nitrous-oxide reductase	polymer	646	71891.5	2	UniProt (P19573) Pfam (PF18793) Pfam (PF18764) Pfam (PF00116)	Pseudomonas stutzeri	N(2)OR,N2O reductase
2	C, D, K (A, B)	FORMIC ACID	non-polymer		46.0	3	Chemie (FMT)
3	E, J (A, B)	2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		282.3	2	Chemie (B3P)
4	F, L (A, B)	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
5	G, M (A, B)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
6	H, N (A, B)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
7	I, O (A, B)	DINUCLEAR COPPER ION	non-polymer		127.1	2	Chemie (CUA)
8	P, Q (A, B)	water	water		18.0	927	Chemie (HOH)

Sequence modifications

A, B: 1 - 638 (UniProt: P19573)

PDB	External Database	Details
Ala 130	His 130	engineered mutation
Trp 639	-	expression tag
Ser 640	-	expression tag
His 641	-	expression tag
Pro 642	-	expression tag
Gln 643	-	expression tag
Phe 644	-	expression tag
Glu 645	-	expression tag
Lys 646	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	143783.0
Non-Polymers*	Number of molecules	13
	Total formula weight	1154.0
All*	Total formula weight	144936.9

*Water molecules are not included.